Differential phosphorylation of Clr4SUV39H by Cdk1 accompanies a histone H3 methylation switch that is essential for gametogenesis
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Differential phosphorylation of Clr4SUV39H by Cdk1 accompanies a histone H3 methylation switch that is essential for gametogenesis. / Kuzdere, Tahsin; Flury, Valentin; Schalch, Thomas; Iesmantavicius, Vytautas; Hess, Daniel; Bühler, Marc.
In: EMBO Reports, Vol. 24, No. 1, e55928, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Differential phosphorylation of Clr4SUV39H by Cdk1 accompanies a histone H3 methylation switch that is essential for gametogenesis
AU - Kuzdere, Tahsin
AU - Flury, Valentin
AU - Schalch, Thomas
AU - Iesmantavicius, Vytautas
AU - Hess, Daniel
AU - Bühler, Marc
N1 - Publisher Copyright: © 2022 Friedrich Miescher Institute for Biomedical Research. Published under the terms of the CC BY 4.0 license.
PY - 2023
Y1 - 2023
N2 - Methylation of histone H3 at lysine 9 (H3K9) is a hallmark of heterochromatin that plays crucial roles in gene silencing, genome stability, and chromosome segregation. In Schizosaccharomyces pombe, Clr4 mediates both di- and tri-methylation of H3K9. Although H3K9 methylation has been intensely studied in mitotic cells, its role during sexual differentiation remains unclear. Here, we map H3K9 methylation genome-wide during meiosis and show that constitutive heterochromatin temporarily loses H3K9me2 and becomes H3K9me3 when cells commit to meiosis. Cells lacking the ability to tri-methylate H3K9 exhibit meiotic chromosome segregation defects. Finally, the H3K9 methylation switch is accompanied by differential phosphorylation of Clr4 by the cyclin-dependent kinase Cdk1. Our results suggest that a conserved master regulator of the cell cycle controls the specificity of an H3K9 methyltransferase to prevent ectopic H3K9 methylation and to ensure faithful gametogenesis.
AB - Methylation of histone H3 at lysine 9 (H3K9) is a hallmark of heterochromatin that plays crucial roles in gene silencing, genome stability, and chromosome segregation. In Schizosaccharomyces pombe, Clr4 mediates both di- and tri-methylation of H3K9. Although H3K9 methylation has been intensely studied in mitotic cells, its role during sexual differentiation remains unclear. Here, we map H3K9 methylation genome-wide during meiosis and show that constitutive heterochromatin temporarily loses H3K9me2 and becomes H3K9me3 when cells commit to meiosis. Cells lacking the ability to tri-methylate H3K9 exhibit meiotic chromosome segregation defects. Finally, the H3K9 methylation switch is accompanied by differential phosphorylation of Clr4 by the cyclin-dependent kinase Cdk1. Our results suggest that a conserved master regulator of the cell cycle controls the specificity of an H3K9 methyltransferase to prevent ectopic H3K9 methylation and to ensure faithful gametogenesis.
KW - chromosome segregation
KW - fission yeast
KW - histone methylation
KW - meiosis
KW - phosphorylation
U2 - 10.15252/embr.202255928
DO - 10.15252/embr.202255928
M3 - Journal article
C2 - 36408846
AN - SCOPUS:85142260411
VL - 24
JO - E M B O Reports
JF - E M B O Reports
SN - 1469-221X
IS - 1
M1 - e55928
ER -
ID: 337385559