Differences in motility pattern between human buccal fibroblasts and periodontal and skin fibroblasts
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Differences in motility pattern between human buccal fibroblasts and periodontal and skin fibroblasts. / Lepekhin, Eugene; Grøn, Birgitte; Berezin, Vladimir; Bock, Elisabeth; Dabelsteen, Erik.
In: European Journal of Oral Sciences, Vol. 110, No. 1, 02.2002, p. 13-20.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Differences in motility pattern between human buccal fibroblasts and periodontal and skin fibroblasts
AU - Lepekhin, Eugene
AU - Grøn, Birgitte
AU - Berezin, Vladimir
AU - Bock, Elisabeth
AU - Dabelsteen, Erik
PY - 2002/2
Y1 - 2002/2
N2 - Migration of fibroblasts from surrounding normal tissue into the wound bed is an important requirement for successful wound healing. This study investigated the motility pattern of buccal, periodontal and skin fibroblasts to determine whether differences in the wound healing efficiency at these sites can be explained by differences in the motile behavior of their respective fibroblast populations. The migratory characteristics were studied in a two-dimensional culture system. The migration of single cells was time-lapse video recorded at intervals of 15 min for a period of 6 h using a computer-assisted microscope work-station. For evaluation of cell morphology, cell contours were recognized semiautomatically and used for determination of cell area, cell spreading and number and length of processes. We found that the cellular displacement of the buccal fibroblasts was only approximately 50% of the cellular displacement of periodontal and skin fibroblasts. The decreased cellular displacement of the buccal fibroblasts was found to be due to both lower cellular speed and less persistence in direction. The buccal fibroblasts also displayed smaller areas and longer processes. The differences in cellular morphology and motility pattern amongst the three fibroblast types could not be explained by differences in secretion of extracellular matrix components and are therefore believed to reflect phenotypic differences amongst fibroblast subpopulations.
AB - Migration of fibroblasts from surrounding normal tissue into the wound bed is an important requirement for successful wound healing. This study investigated the motility pattern of buccal, periodontal and skin fibroblasts to determine whether differences in the wound healing efficiency at these sites can be explained by differences in the motile behavior of their respective fibroblast populations. The migratory characteristics were studied in a two-dimensional culture system. The migration of single cells was time-lapse video recorded at intervals of 15 min for a period of 6 h using a computer-assisted microscope work-station. For evaluation of cell morphology, cell contours were recognized semiautomatically and used for determination of cell area, cell spreading and number and length of processes. We found that the cellular displacement of the buccal fibroblasts was only approximately 50% of the cellular displacement of periodontal and skin fibroblasts. The decreased cellular displacement of the buccal fibroblasts was found to be due to both lower cellular speed and less persistence in direction. The buccal fibroblasts also displayed smaller areas and longer processes. The differences in cellular morphology and motility pattern amongst the three fibroblast types could not be explained by differences in secretion of extracellular matrix components and are therefore believed to reflect phenotypic differences amongst fibroblast subpopulations.
KW - Actins
KW - Adult
KW - Algorithms
KW - Cell Count
KW - Cell Culture Techniques
KW - Cell Movement
KW - Cell Size
KW - Collagen Type I
KW - Coloring Agents
KW - Extracellular Matrix
KW - Fibroblasts
KW - Humans
KW - Image Processing, Computer-Assisted
KW - Immunohistochemistry
KW - Microscopy, Video
KW - Mouth Mucosa
KW - Periodontal Ligament
KW - Skin
KW - Statistics as Topic
KW - Time Factors
KW - Video Recording
KW - Wound Healing
M3 - Journal article
C2 - 11878755
VL - 110
SP - 13
EP - 20
JO - European Journal of Oral Sciences
JF - European Journal of Oral Sciences
SN - 0909-8836
IS - 1
ER -
ID: 119593525