Determination of membrane protein orientation upon liposomal reconstitution down to the single vesicle level
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Accepted author manuscript, 1.8 MB, PDF document
Reconstitution of membrane proteins into liposomal membranes represents a key technique in enabling functional analysis under well-defined conditions. In this review, we provide a brief introduction to selected methods that have been developed to determine membrane protein orientation after reconstitution in liposomes, including approaches based on proteolytic digestion with proteases, site-specific labeling, fluorescence quenching and activity assays. In addition, we briefly highlight new strategies based on single vesicle analysis to address the problem of sample heterogeneity.
Original language | English |
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Journal | Biological Chemistry |
Volume | 404 |
Issue number | 7 |
ISSN | 1431-6730 |
DOIs | |
Publication status | Published - 2023 |
- membrane protein, protease, proteoliposome, quencher, reconstitution, single vesicle, ORIENTED INSERTION, LIPID-COMPOSITION, FUSION PROTEINS, PH CHANGES, BACTERIORHODOPSIN, SITE, TRANSLOCATION, MECHANISMS, MOLECULES, TRANSPORT
Research areas
ID: 339139966