Detection of Clostridium botulinum neurotoxin genes (A-F) in dairy farms from Northern Germany using PCR: A case-control study
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Detection of Clostridium botulinum neurotoxin genes (A-F) in dairy farms from Northern Germany using PCR : A case-control study. / Fohler, Svenja; Discher, Sabrina; Jordan, Eva; Seyboldt, Christian; Klein, Guenter; Neubauer, Heinrich; Hoedemaker, Martina; Scheu, Theresa; Campe, Amely; Jensen, Katharina ; Abdulmawjood, Amir.
In: Anaerobe, Vol. 39, 06.2016, p. 97-104.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Detection of Clostridium botulinum neurotoxin genes (A-F) in dairy farms from Northern Germany using PCR
T2 - A case-control study
AU - Fohler, Svenja
AU - Discher, Sabrina
AU - Jordan, Eva
AU - Seyboldt, Christian
AU - Klein, Guenter
AU - Neubauer, Heinrich
AU - Hoedemaker, Martina
AU - Scheu, Theresa
AU - Campe, Amely
AU - Jensen, Katharina
AU - Abdulmawjood, Amir
N1 - Copyright © 2016 Elsevier Ltd. All rights reserved.
PY - 2016/6
Y1 - 2016/6
N2 - Classical botulism in cattle mainly occurs after ingestion of feed contaminated with preformed toxin. In 2001 a form of botulism ("visceral botulism") was postulated to occur after ingestion of Clostridium (C.) botulinum cells or spores, followed by colonization of the intestine, and local production of botulinum neurotoxin (BoNT) causing chronic generalized disease. To verify the potential role of C. botulinum in the described syndrome, a case-control study was conducted, including 139 farms. Fecal samples, rumen content, water and silage samples were collected on each farm. Real time BoNT gene PCR assays were conducted after enrichment in RCM (Reinforced Clostridial Medium) at 37 °C and conventional PCRs after enrichment in MCM (Modified Cooked Meat Medium) at 30 °C. Furthermore, a direct detection of BoNT genes without prior enrichment was attempted. BoNT A, B, C, D, E and F genes were detected in animal samples from 25 (17.99%), 3 (2.16%), 0 (0.0%), 2 (1.44%), 1 (0.72%), and 3 (2.16%) farms, respectively. Eleven feed samples were positive for BoNT A gene. By enrichment a significant increase in sensitivity was achieved. Therefore, this should be an essential part of any protocol. No significant differences regarding BoNT gene occurrence could be observed between Case and Control farms or chronically diseased and clinically healthy animals within the particular category. Thus, the postulated form of chronic botulism in cows could not be confirmed. This study supports the general opinion that C. botulinum can occasionally be found in the rumen and intestine of cows without causing disease.
AB - Classical botulism in cattle mainly occurs after ingestion of feed contaminated with preformed toxin. In 2001 a form of botulism ("visceral botulism") was postulated to occur after ingestion of Clostridium (C.) botulinum cells or spores, followed by colonization of the intestine, and local production of botulinum neurotoxin (BoNT) causing chronic generalized disease. To verify the potential role of C. botulinum in the described syndrome, a case-control study was conducted, including 139 farms. Fecal samples, rumen content, water and silage samples were collected on each farm. Real time BoNT gene PCR assays were conducted after enrichment in RCM (Reinforced Clostridial Medium) at 37 °C and conventional PCRs after enrichment in MCM (Modified Cooked Meat Medium) at 30 °C. Furthermore, a direct detection of BoNT genes without prior enrichment was attempted. BoNT A, B, C, D, E and F genes were detected in animal samples from 25 (17.99%), 3 (2.16%), 0 (0.0%), 2 (1.44%), 1 (0.72%), and 3 (2.16%) farms, respectively. Eleven feed samples were positive for BoNT A gene. By enrichment a significant increase in sensitivity was achieved. Therefore, this should be an essential part of any protocol. No significant differences regarding BoNT gene occurrence could be observed between Case and Control farms or chronically diseased and clinically healthy animals within the particular category. Thus, the postulated form of chronic botulism in cows could not be confirmed. This study supports the general opinion that C. botulinum can occasionally be found in the rumen and intestine of cows without causing disease.
KW - Animals
KW - Botulinum Toxins, Type A/genetics
KW - Botulism/microbiology
KW - Case-Control Studies
KW - Cattle
KW - Clostridium botulinum/isolation & purification
KW - Dairying
KW - Drinking Water/chemistry
KW - Farms
KW - Feces/chemistry
KW - Female
KW - Germany
KW - Polymerase Chain Reaction
KW - Protein Isoforms/genetics
KW - Rumen/chemistry
KW - Silage/analysis
U2 - 10.1016/j.anaerobe.2016.03.008
DO - 10.1016/j.anaerobe.2016.03.008
M3 - Journal article
C2 - 27016061
VL - 39
SP - 97
EP - 104
JO - Anaerobe
JF - Anaerobe
SN - 1075-9964
ER -
ID: 368799319