Delivery of endocytosed proteins to the cell-division plane requires change of pathway from recycling to secretion
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Delivery of endocytosed proteins to the cell-division plane requires change of pathway from recycling to secretion. / Richter, Sandra; Kientz, Marika; Brumm, Sabine; Nielsen, Mads Eggert; Park, Misoon; Gavidia, Richard; Krause, Cornelia; Voss, Ute; Beckmann, Hauke; Mayer, Ulrike; Stierhof, York Dieter; Jürgens, Gerd.
In: eLife, Vol. 2014, No. 3, e02131, 2014.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Delivery of endocytosed proteins to the cell-division plane requires change of pathway from recycling to secretion
AU - Richter, Sandra
AU - Kientz, Marika
AU - Brumm, Sabine
AU - Nielsen, Mads Eggert
AU - Park, Misoon
AU - Gavidia, Richard
AU - Krause, Cornelia
AU - Voss, Ute
AU - Beckmann, Hauke
AU - Mayer, Ulrike
AU - Stierhof, York Dieter
AU - Jürgens, Gerd
PY - 2014
Y1 - 2014
N2 - Membrane trafficking is essential to fundamental processes in eukaryotic life, including cell growth and division. In plant cytokinesis, post-Golgi trafficking mediates a massive flow of vesicles that form the partitioning membrane but its regulation remains poorly understood. Here, we identify functionally redundant Arabidopsis ARF guanine-nucleotide exchange factors (ARF-GEFs) BIG1-BIG4 as regulators of post-Golgi trafficking, mediating late secretion from the trans- Golgi network but not recycling of endocytosed proteins to the plasma membrane, although the TGN also functions as an early endosome in plants. In contrast, BIG1-4 are absolutely required for trafficking of both endocytosed and newly synthesized proteins to the cell-division plane during cytokinesis, counteracting recycling to the plasma membrane. This change from recycling to secretory trafficking pathway mediated by ARF-GEFs confers specificity of cargo delivery to the division plane and might thus ensure that the partitioning membrane is completed on time in the absence of a cytokinesis-interphase checkpoint.
AB - Membrane trafficking is essential to fundamental processes in eukaryotic life, including cell growth and division. In plant cytokinesis, post-Golgi trafficking mediates a massive flow of vesicles that form the partitioning membrane but its regulation remains poorly understood. Here, we identify functionally redundant Arabidopsis ARF guanine-nucleotide exchange factors (ARF-GEFs) BIG1-BIG4 as regulators of post-Golgi trafficking, mediating late secretion from the trans- Golgi network but not recycling of endocytosed proteins to the plasma membrane, although the TGN also functions as an early endosome in plants. In contrast, BIG1-4 are absolutely required for trafficking of both endocytosed and newly synthesized proteins to the cell-division plane during cytokinesis, counteracting recycling to the plasma membrane. This change from recycling to secretory trafficking pathway mediated by ARF-GEFs confers specificity of cargo delivery to the division plane and might thus ensure that the partitioning membrane is completed on time in the absence of a cytokinesis-interphase checkpoint.
U2 - 10.7554/eLife.02131
DO - 10.7554/eLife.02131
M3 - Journal article
C2 - 24714496
AN - SCOPUS:84898471427
VL - 2014
JO - eLife
JF - eLife
SN - 2050-084X
IS - 3
M1 - e02131
ER -
ID: 130101593