TY - JOUR

T1 - Deciphering dynamic dose responses of natural promoters and single cis elements upon osmotic and oxidative stress in yeast

AU - Dolz-Edo, Laura

AU - Rienzo, Alessandro

AU - Poveda-Huertes, Daniel

AU - Pascual-Ahuir, Amparo

AU - Proft, Markus

PY - 2013/6

Y1 - 2013/6

N2 - Fine-tuned activation of gene expression in response to stress is the result of dynamic interactions of transcription factors with specific promoter binding sites. In the study described here we used a time-resolved luciferase reporter assay in living Saccharomyces cerevisiae yeast cells to gain insights into how osmotic and oxidative stress signals modulate gene expression in a dose-sensitive manner. Specifically, the dose-response behavior of four different natural promoters (GRE2, CTT1, SOD2, and CCP1) reveals differences in their sensitivity and dynamics in response to different salt and oxidative stimuli. Characteristic dose-response profiles were also obtained for artificial promoters driven by only one type of stress-regulated consensus element, such as the cyclic AMP-responsive element, stress response element, or AP-1 site. Oxidative and osmotic stress signals activate these elements separately and with different sensitivities through different signaling molecules. Combination of stress-activated cis elements does not, in general, enhance the absolute expression levels; however, specific combinations can increase the inducibility of the promoter in response to different stress doses. Finally, we show that the stress tolerance of the cell critically modulates the dynamics of its transcriptional response in the case of oxidative stress.

AB - Fine-tuned activation of gene expression in response to stress is the result of dynamic interactions of transcription factors with specific promoter binding sites. In the study described here we used a time-resolved luciferase reporter assay in living Saccharomyces cerevisiae yeast cells to gain insights into how osmotic and oxidative stress signals modulate gene expression in a dose-sensitive manner. Specifically, the dose-response behavior of four different natural promoters (GRE2, CTT1, SOD2, and CCP1) reveals differences in their sensitivity and dynamics in response to different salt and oxidative stimuli. Characteristic dose-response profiles were also obtained for artificial promoters driven by only one type of stress-regulated consensus element, such as the cyclic AMP-responsive element, stress response element, or AP-1 site. Oxidative and osmotic stress signals activate these elements separately and with different sensitivities through different signaling molecules. Combination of stress-activated cis elements does not, in general, enhance the absolute expression levels; however, specific combinations can increase the inducibility of the promoter in response to different stress doses. Finally, we show that the stress tolerance of the cell critically modulates the dynamics of its transcriptional response in the case of oxidative stress.

KW - Base Sequence

KW - Basic-Leucine Zipper Transcription Factors/genetics

KW - DNA-Binding Proteins/genetics

KW - Gene Expression Regulation, Fungal

KW - Mitogen-Activated Protein Kinases/genetics

KW - Molecular Sequence Data

KW - Osmosis

KW - Oxidative Stress/genetics

KW - Oxidoreductases/genetics

KW - Promoter Regions, Genetic

KW - Regulatory Sequences, Nucleic Acid

KW - Repressor Proteins/genetics

KW - Saccharomyces cerevisiae/physiology

KW - Saccharomyces cerevisiae Proteins/genetics

KW - Salt Tolerance/genetics

KW - Superoxide Dismutase/genetics

KW - Transcription Factor AP-1/genetics

KW - Transcription Factors/genetics

U2 - 10.1128/MCB.00240-13

DO - 10.1128/MCB.00240-13

M3 - Journal article

C2 - 23530054

VL - 33

SP - 2228

EP - 2240

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 11

ER -