Cytokine production by virus-specific CD8(+) T cells varies with activation state and localization, but not with TCR avidity
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Cytokine production by virus-specific CD8(+) T cells varies with activation state and localization, but not with TCR avidity. / Kristensen, Nanna Ny; Madsen, Andreas Nygaard; Thomsen, Allan Randrup; Christensen, Jan Pravsgaard.
In: Journal of General Virology, Vol. 85, No. Pt 6, 2004, p. 1703-12.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cytokine production by virus-specific CD8(+) T cells varies with activation state and localization, but not with TCR avidity
AU - Kristensen, Nanna Ny
AU - Madsen, Andreas Nygaard
AU - Thomsen, Allan Randrup
AU - Christensen, Jan Pravsgaard
N1 - Keywords: Animals; CD8-Positive T-Lymphocytes; Cytokines; Female; Granulocyte-Macrophage Colony-Stimulating Factor; Immunologic Memory; Interferon-gamma; Lymphocyte Activation; Lymphocytic choriomeningitis virus; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Receptors, Antigen, T-Cell; Tumor Necrosis Factor-alpha; Vesicular stomatitis Indiana virus
PY - 2004
Y1 - 2004
N2 - The ability of virus-specific CD8(+) T cells to produce cytokines was studied in mice infected with lymphocytic choriomeningitis virus and vesicular stomatitis virus. Intracellular staining was used to visualize cytokine-producing CD8(+) and CD4(+) T cells. Overall, virus-specific CD8(+) T cells produce a similar range of cytokines (IFN-gamma, TNF-alpha, IL-2, GM-CSF, RANTES, MIP-1alpha and MIP-1beta) as CD4(+) T cells, but the relative distribution of cytokine-producing subsets is different. Moreover, cytokine-producing CD8(+) T cells were found to dominate numerically at all time-points tested. Co-staining for more than one cytokine revealed that while all cytokine-producing CD8(+) T cells synthesized IFN-gamma, additional cytokines were produced by partly overlapping subsets of this population. The frequency of cells producing more than one cytokine was higher in a tertiary site (peritoneum) and generally increased with transition into the memory phase; however, GM-CSF producing cells were only present transiently. Concerning factors predicted to influence the distribution of cytokine-producing subsets, IFN-gamma and IL-12 did not play a role, nor was extensive virus replication essential. Notably, regarding the heterogeneity in cytokine production by individual cells with similar epitope specificity, variation in TCR avidity was not the cause, since in vivo-activated TCR transgene-expressing cells were as heterogeneous in cytokine expression as polyclonal cells specific for the same epitope.
AB - The ability of virus-specific CD8(+) T cells to produce cytokines was studied in mice infected with lymphocytic choriomeningitis virus and vesicular stomatitis virus. Intracellular staining was used to visualize cytokine-producing CD8(+) and CD4(+) T cells. Overall, virus-specific CD8(+) T cells produce a similar range of cytokines (IFN-gamma, TNF-alpha, IL-2, GM-CSF, RANTES, MIP-1alpha and MIP-1beta) as CD4(+) T cells, but the relative distribution of cytokine-producing subsets is different. Moreover, cytokine-producing CD8(+) T cells were found to dominate numerically at all time-points tested. Co-staining for more than one cytokine revealed that while all cytokine-producing CD8(+) T cells synthesized IFN-gamma, additional cytokines were produced by partly overlapping subsets of this population. The frequency of cells producing more than one cytokine was higher in a tertiary site (peritoneum) and generally increased with transition into the memory phase; however, GM-CSF producing cells were only present transiently. Concerning factors predicted to influence the distribution of cytokine-producing subsets, IFN-gamma and IL-12 did not play a role, nor was extensive virus replication essential. Notably, regarding the heterogeneity in cytokine production by individual cells with similar epitope specificity, variation in TCR avidity was not the cause, since in vivo-activated TCR transgene-expressing cells were as heterogeneous in cytokine expression as polyclonal cells specific for the same epitope.
M3 - Journal article
C2 - 15166455
VL - 85
SP - 1703
EP - 1712
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - Pt 6
ER -
ID: 9639266