CTD-dependent and -independent mechanisms govern co-transcriptional capping of Pol II transcripts
Research output: Contribution to journal › Journal article › Research › peer-review
Co-transcriptional capping of RNA polymerase II (Pol II) transcripts by capping enzyme proceeds orders of magnitude more efficiently than capping of free RNA. Previous studies brought to light a role for the phosphorylated Pol II carboxyl-terminal domain (CTD) in activation of co-transcriptional capping; however, CTD phosphorylation alone could not account for the observed magnitude of activation. Here, we exploit a defined Pol II transcription system that supports both CTD phosphorylation and robust activation of capping to dissect the mechanism of co-transcriptional capping. Taken together, our findings identify a CTD-independent, but Pol II-mediated, mechanism that functions in parallel with CTD-dependent processes to ensure optimal capping, and they support a "tethering" model for the mechanism of activation.
Original language | English |
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Journal | Nature Communications |
Volume | 9 |
Issue number | 1 |
Pages (from-to) | 3392 |
ISSN | 2041-1723 |
DOIs | |
Publication status | Published - 23 Aug 2018 |
Externally published | Yes |
- Base Sequence, Cyclin-Dependent Kinases/metabolism, Humans, Models, Biological, Phosphorylation, Protein Domains, RNA Caps/metabolism, RNA Polymerase II/chemistry, RNA, Messenger/genetics, Species Specificity, Structure-Activity Relationship, Transcription Factor TFIIH/metabolism, Transcription, Genetic
Research areas
ID: 262753257