CTD-dependent and -independent mechanisms govern co-transcriptional capping of Pol II transcripts

Research output: Contribution to journal › Journal article › Research › peer-review

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CTD-dependent and -independent mechanisms govern co-transcriptional capping of Pol II transcripts. / Noe Gonzalez, Melvin; Sato, Shigeo; Tomomori-Sato, Chieri; Conaway, Joan W; Conaway, Ronald C.

In: Nature Communications, Vol. 9, No. 1, 23.08.2018, p. 3392.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Noe Gonzalez, M, Sato, S, Tomomori-Sato, C, Conaway, JW & Conaway, RC 2018, 'CTD-dependent and -independent mechanisms govern co-transcriptional capping of Pol II transcripts', Nature Communications, vol. 9, no. 1, pp. 3392. https://doi.org/10.1038/s41467-018-05923-w

APA

Noe Gonzalez, M., Sato, S., Tomomori-Sato, C., Conaway, J. W., & Conaway, R. C. (2018). CTD-dependent and -independent mechanisms govern co-transcriptional capping of Pol II transcripts. Nature Communications, 9(1), 3392. https://doi.org/10.1038/s41467-018-05923-w

Vancouver

Noe Gonzalez M, Sato S, Tomomori-Sato C, Conaway JW, Conaway RC. CTD-dependent and -independent mechanisms govern co-transcriptional capping of Pol II transcripts. Nature Communications. 2018 Aug 23;9(1):3392. https://doi.org/10.1038/s41467-018-05923-w

Author

Noe Gonzalez, Melvin ; Sato, Shigeo ; Tomomori-Sato, Chieri ; Conaway, Joan W ; Conaway, Ronald C. / CTD-dependent and -independent mechanisms govern co-transcriptional capping of Pol II transcripts. In: Nature Communications. 2018 ; Vol. 9, No. 1. pp. 3392.

Bibtex

@article{1e94713847384d8c812e4511ae547db9,
title = "CTD-dependent and -independent mechanisms govern co-transcriptional capping of Pol II transcripts",
abstract = "Co-transcriptional capping of RNA polymerase II (Pol II) transcripts by capping enzyme proceeds orders of magnitude more efficiently than capping of free RNA. Previous studies brought to light a role for the phosphorylated Pol II carboxyl-terminal domain (CTD) in activation of co-transcriptional capping; however, CTD phosphorylation alone could not account for the observed magnitude of activation. Here, we exploit a defined Pol II transcription system that supports both CTD phosphorylation and robust activation of capping to dissect the mechanism of co-transcriptional capping. Taken together, our findings identify a CTD-independent, but Pol II-mediated, mechanism that functions in parallel with CTD-dependent processes to ensure optimal capping, and they support a {"}tethering{"} model for the mechanism of activation.",
keywords = "Base Sequence, Cyclin-Dependent Kinases/metabolism, Humans, Models, Biological, Phosphorylation, Protein Domains, RNA Caps/metabolism, RNA Polymerase II/chemistry, RNA, Messenger/genetics, Species Specificity, Structure-Activity Relationship, Transcription Factor TFIIH/metabolism, Transcription, Genetic",
author = "{Noe Gonzalez}, Melvin and Shigeo Sato and Chieri Tomomori-Sato and Conaway, {Joan W} and Conaway, {Ronald C}",
year = "2018",
month = aug,
day = "23",
doi = "10.1038/s41467-018-05923-w",
language = "English",
volume = "9",
pages = "3392",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - CTD-dependent and -independent mechanisms govern co-transcriptional capping of Pol II transcripts

AU - Noe Gonzalez, Melvin

AU - Sato, Shigeo

AU - Tomomori-Sato, Chieri

AU - Conaway, Joan W

AU - Conaway, Ronald C

PY - 2018/8/23

Y1 - 2018/8/23

N2 - Co-transcriptional capping of RNA polymerase II (Pol II) transcripts by capping enzyme proceeds orders of magnitude more efficiently than capping of free RNA. Previous studies brought to light a role for the phosphorylated Pol II carboxyl-terminal domain (CTD) in activation of co-transcriptional capping; however, CTD phosphorylation alone could not account for the observed magnitude of activation. Here, we exploit a defined Pol II transcription system that supports both CTD phosphorylation and robust activation of capping to dissect the mechanism of co-transcriptional capping. Taken together, our findings identify a CTD-independent, but Pol II-mediated, mechanism that functions in parallel with CTD-dependent processes to ensure optimal capping, and they support a "tethering" model for the mechanism of activation.

AB - Co-transcriptional capping of RNA polymerase II (Pol II) transcripts by capping enzyme proceeds orders of magnitude more efficiently than capping of free RNA. Previous studies brought to light a role for the phosphorylated Pol II carboxyl-terminal domain (CTD) in activation of co-transcriptional capping; however, CTD phosphorylation alone could not account for the observed magnitude of activation. Here, we exploit a defined Pol II transcription system that supports both CTD phosphorylation and robust activation of capping to dissect the mechanism of co-transcriptional capping. Taken together, our findings identify a CTD-independent, but Pol II-mediated, mechanism that functions in parallel with CTD-dependent processes to ensure optimal capping, and they support a "tethering" model for the mechanism of activation.

KW - Base Sequence

KW - Cyclin-Dependent Kinases/metabolism

KW - Humans

KW - Models, Biological

KW - Phosphorylation

KW - Protein Domains

KW - RNA Caps/metabolism

KW - RNA Polymerase II/chemistry

KW - RNA, Messenger/genetics

KW - Species Specificity

KW - Structure-Activity Relationship

KW - Transcription Factor TFIIH/metabolism

KW - Transcription, Genetic

U2 - 10.1038/s41467-018-05923-w

DO - 10.1038/s41467-018-05923-w

M3 - Journal article

C2 - 30139934

VL - 9

SP - 3392

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

ER -

ID: 262753257