Cross-infection of virulent Dichelobacter nodosus between sheep and co-grazing cattle
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Cross-infection of virulent Dichelobacter nodosus between sheep and co-grazing cattle. / Knappe-Poindecker, Maren; Gilhuus, Marianne; Jensen, Tim Kåre; Vatn, Synnøve; Jørgensen, Hannah J.; Fjeldaas, Terje.
In: Veterinary Microbiology, 2014.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cross-infection of virulent Dichelobacter nodosus between sheep and co-grazing cattle
AU - Knappe-Poindecker, Maren
AU - Gilhuus, Marianne
AU - Jensen, Tim Kåre
AU - Vatn, Synnøve
AU - Jørgensen, Hannah J.
AU - Fjeldaas, Terje
PY - 2014
Y1 - 2014
N2 - Dichelobacter nodosus is the main aetiological agent of ovine footrot and the bacterium has also been associated with interdigital dermatitis is cattle. The aim of this study was to investigate possible cross-infection of virulent D. nodosus between sheep and co-grazing cattle. Five farms, where sheep previously diagnosed with virulent D. nodosus were co-grazing with cattle for different periods of time, were included. The study sample consisted of 200 cows and 50 sheep. All cows were examined for the presence of interdigital dermatitis, and ten ewes, preferably with symptoms of footrot, had the footrot scores recorded. On each farm, the same ten ewes and ten cows were chosen for bacterial analyses. Swabs were analysed for D. nodosus by PCR and culturing. D. nodosus isolates were virulence-tested and assigned to serogroups by fimA variant determination. Biopsies were evaluated histopathologically and analysed by fluorescent in situ hybridization for D. nodosus, Treponema spp. and Fusobacterium necrophorum. D. nodosus defined as virulent by the gelatin gel test were isolated from 16 sheep from four farms and from five cows from two of the same farms. All five cows had interdigital dermatitis. Two of the cows stayed infected for at least eight months. By pulsed-field gel electrophoresis (PFGE), the isolates from the five cows were found to be genetically indistinguishable or closely related to isolates from sheep from the same farm. This indicates that cross-infection between sheep and cows have occurred.
AB - Dichelobacter nodosus is the main aetiological agent of ovine footrot and the bacterium has also been associated with interdigital dermatitis is cattle. The aim of this study was to investigate possible cross-infection of virulent D. nodosus between sheep and co-grazing cattle. Five farms, where sheep previously diagnosed with virulent D. nodosus were co-grazing with cattle for different periods of time, were included. The study sample consisted of 200 cows and 50 sheep. All cows were examined for the presence of interdigital dermatitis, and ten ewes, preferably with symptoms of footrot, had the footrot scores recorded. On each farm, the same ten ewes and ten cows were chosen for bacterial analyses. Swabs were analysed for D. nodosus by PCR and culturing. D. nodosus isolates were virulence-tested and assigned to serogroups by fimA variant determination. Biopsies were evaluated histopathologically and analysed by fluorescent in situ hybridization for D. nodosus, Treponema spp. and Fusobacterium necrophorum. D. nodosus defined as virulent by the gelatin gel test were isolated from 16 sheep from four farms and from five cows from two of the same farms. All five cows had interdigital dermatitis. Two of the cows stayed infected for at least eight months. By pulsed-field gel electrophoresis (PFGE), the isolates from the five cows were found to be genetically indistinguishable or closely related to isolates from sheep from the same farm. This indicates that cross-infection between sheep and cows have occurred.
UR - https://orbit.dtu.dk/en/publications/d712ff6c-eb7c-4323-a583-274f965da206
U2 - 10.1016/j.vetmic.2014.02.044
DO - 10.1016/j.vetmic.2014.02.044
M3 - Journal article
C2 - 24698131
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
ER -
ID: 339251435