Comparing proteome changes involved in biofilm formation by Streptococcus mutans after exposure to sucrose and starch
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Comparing proteome changes involved in biofilm formation by Streptococcus mutans after exposure to sucrose and starch. / Rezaei, Tohid; Kamounah, Fadhil S.; Khodadadi, Ehsaneh; Mehramouz, Bahareh; Gholizadeh, Pourya; Yousefi, Leila; Ganbarov, Khudaverdi; Ghotaslou, Reza; Yousefi, Mehdi; Asgharzadeh, Mohammad; Eslami, Hosein; Taghizadeh, Sepehr; Pirzadeh, Tahereh; Kafil, Hossein Samadi.
In: Biotechnology and Applied Biochemistry, Vol. 70, No. 3, 2023, p. 1320-1331.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Comparing proteome changes involved in biofilm formation by Streptococcus mutans after exposure to sucrose and starch
AU - Rezaei, Tohid
AU - Kamounah, Fadhil S.
AU - Khodadadi, Ehsaneh
AU - Mehramouz, Bahareh
AU - Gholizadeh, Pourya
AU - Yousefi, Leila
AU - Ganbarov, Khudaverdi
AU - Ghotaslou, Reza
AU - Yousefi, Mehdi
AU - Asgharzadeh, Mohammad
AU - Eslami, Hosein
AU - Taghizadeh, Sepehr
AU - Pirzadeh, Tahereh
AU - Kafil, Hossein Samadi
N1 - Funding Information: This study was supported by Drug Applied Research Center with grant number 67426 and approved by local ethics committee with reference number IR.TBZMED.VCR.REC.1400.187. Publisher Copyright: © 2023 International Union of Biochemistry and Molecular Biology, Inc.
PY - 2023
Y1 - 2023
N2 - Streptococcus mutans is a main organism of tooth infections including tooth decay and periodontitis. The aim of this study was to assess the influence of sucrose and starch on biofilm formation and proteome profile of S. mutans ATCC 35668 strain. The biofilm formation was assessed by microtiter plating method. Changes in bacterial proteins after exposure to sucrose and starch carbohydrates were analyzed using matrix-assisted laser desorption/ionization mass spectrometry. The biofilm formation of S. mutans was increased to 391.76% in 1% sucrose concentration, 165.76% in 1% starch, and 264.27% in the 0.5% sucrose plus 0.5% starch in comparison to biofilm formation in the media without sugars. The abundance of glutamines, adenylate kinase, and 50S ribosomal protein L29 was increased under exposure to sucrose. Upregulation of lactate utilization protein C, 5-hydroxybenzimidazole synthase BzaA, and 50S ribosomal protein L16 was formed under starch exposure. Ribosome-recycling factor, peptide chain release factor 1, and peptide methionine sulfoxide reductase MsrB were upregulated under exposure to sucrose in combination with starch. The results demonstrated that the carbohydrates increase microbial pathogenicity. In addition, sucrose and starch carbohydrates can induce biofilm formation of S. mutans via various mechanisms such as changes in the expression of special proteins.
AB - Streptococcus mutans is a main organism of tooth infections including tooth decay and periodontitis. The aim of this study was to assess the influence of sucrose and starch on biofilm formation and proteome profile of S. mutans ATCC 35668 strain. The biofilm formation was assessed by microtiter plating method. Changes in bacterial proteins after exposure to sucrose and starch carbohydrates were analyzed using matrix-assisted laser desorption/ionization mass spectrometry. The biofilm formation of S. mutans was increased to 391.76% in 1% sucrose concentration, 165.76% in 1% starch, and 264.27% in the 0.5% sucrose plus 0.5% starch in comparison to biofilm formation in the media without sugars. The abundance of glutamines, adenylate kinase, and 50S ribosomal protein L29 was increased under exposure to sucrose. Upregulation of lactate utilization protein C, 5-hydroxybenzimidazole synthase BzaA, and 50S ribosomal protein L16 was formed under starch exposure. Ribosome-recycling factor, peptide chain release factor 1, and peptide methionine sulfoxide reductase MsrB were upregulated under exposure to sucrose in combination with starch. The results demonstrated that the carbohydrates increase microbial pathogenicity. In addition, sucrose and starch carbohydrates can induce biofilm formation of S. mutans via various mechanisms such as changes in the expression of special proteins.
KW - biofilm
KW - carbohydrate
KW - proteome
KW - Streptococcus mutans
U2 - 10.1002/bab.2442
DO - 10.1002/bab.2442
M3 - Journal article
C2 - 36588392
AN - SCOPUS:85145684835
VL - 70
SP - 1320
EP - 1331
JO - Biotechnology and Applied Biochemistry
JF - Biotechnology and Applied Biochemistry
SN - 0885-4513
IS - 3
ER -
ID: 389412719