Column-switching high-performance liquid chromatographic assay for the determination of quercetin in human urine with ultraviolet absorbance detection
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Column-switching high-performance liquid chromatographic assay for the determination of quercetin in human urine with ultraviolet absorbance detection. / Nielsen, Salka E; Dragsted, Lars Ove.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 707, No. 1-2, 1998, p. 81-89.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Column-switching high-performance liquid chromatographic assay for the determination of quercetin in human urine with ultraviolet absorbance detection
AU - Nielsen, Salka E
AU - Dragsted, Lars Ove
N1 - (Ekstern)
PY - 1998
Y1 - 1998
N2 - A high-performance liquid chromatographic method is described for the determination of quercetin in human urine using column-switching and ultraviolet (UV) absorbance detection. Urine samples were enzymatically hydrolysed and solid-phase extracted prior to injection onto the high- performance liquid chromatography (HPLC) system. Prior to elution of quercetin and the internal standard, fisetin, from the first column used for sample clean-up, the six-port valve was switched to the second column for analysis with UV detection. Detection of quercetin was accurate and reproducible, with a detection limit of 5 ng/ml. The method was applied to determine the urinary level of quercetin in 120 samples from an intervention study with fruit juice.
AB - A high-performance liquid chromatographic method is described for the determination of quercetin in human urine using column-switching and ultraviolet (UV) absorbance detection. Urine samples were enzymatically hydrolysed and solid-phase extracted prior to injection onto the high- performance liquid chromatography (HPLC) system. Prior to elution of quercetin and the internal standard, fisetin, from the first column used for sample clean-up, the six-port valve was switched to the second column for analysis with UV detection. Detection of quercetin was accurate and reproducible, with a detection limit of 5 ng/ml. The method was applied to determine the urinary level of quercetin in 120 samples from an intervention study with fruit juice.
KW - Quercetin
UR - http://www.scopus.com/inward/record.url?scp=0032502861&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(97)00574-4
DO - 10.1016/S0378-4347(97)00574-4
M3 - Journal article
C2 - 9613936
AN - SCOPUS:0032502861
VL - 707
SP - 81
EP - 89
JO - Journal of Chromatography - Biomedical Applications
JF - Journal of Chromatography - Biomedical Applications
SN - 0378-4347
IS - 1-2
ER -
ID: 254770881