Colon anastomotic leakage: improving the mouse model
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Colon anastomotic leakage : improving the mouse model. / Pommergaard, Hans-Christian; Achiam, Michael Patrick; Rosenberg, Jacob.
In: Surgery Today, Vol. 44, No. 5, 2014, p. 933-939.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Colon anastomotic leakage
T2 - improving the mouse model
AU - Pommergaard, Hans-Christian
AU - Achiam, Michael Patrick
AU - Rosenberg, Jacob
PY - 2014
Y1 - 2014
N2 - PURPOSE: Colon anastomotic leakage remains a serious and common surgical complication. Animal models are valuable to determine the pathophysiological mechanisms and to evaluate possible methods of prevention. The aim of this study was to develop an optimal model of clinical colon anastomotic leakage in a technically insufficient anastomosis in the mouse.METHODS: A total of 110 mice were used in three pilot studies (1-3) and two experiments (A, B). Due to the high complication rates, the analgesic regimen and surgical techniques were changed throughout the pilot studies/experiments. In the final successful experiment (B), eight and four absorbable sutures were used in the control and intervention anastomoses, respectively, and buprenorphine in chocolate spread was used for pain treatment.RESULTS: In the final model (experiment B), significantly more animals in the intervention group had clinical anastomotic leakage compared with controls (40 vs. 0 %, p = 0.003). The weight loss was greater and the wellness score was also lower in these animals (p < 0.001). The breaking strength of the anastomoses was not significantly different between the control group [0.55 N ± 0.09] and intervention group [0.49 N ± 0.15] (p = 0.091).CONCLUSIONS: This mouse model closely mimics clinical colon anastomotic leakage in humans. The model is of high clinical relevance, since anastomotic leakage has a similar cause, incidence and manifestations in humans.
AB - PURPOSE: Colon anastomotic leakage remains a serious and common surgical complication. Animal models are valuable to determine the pathophysiological mechanisms and to evaluate possible methods of prevention. The aim of this study was to develop an optimal model of clinical colon anastomotic leakage in a technically insufficient anastomosis in the mouse.METHODS: A total of 110 mice were used in three pilot studies (1-3) and two experiments (A, B). Due to the high complication rates, the analgesic regimen and surgical techniques were changed throughout the pilot studies/experiments. In the final successful experiment (B), eight and four absorbable sutures were used in the control and intervention anastomoses, respectively, and buprenorphine in chocolate spread was used for pain treatment.RESULTS: In the final model (experiment B), significantly more animals in the intervention group had clinical anastomotic leakage compared with controls (40 vs. 0 %, p = 0.003). The weight loss was greater and the wellness score was also lower in these animals (p < 0.001). The breaking strength of the anastomoses was not significantly different between the control group [0.55 N ± 0.09] and intervention group [0.49 N ± 0.15] (p = 0.091).CONCLUSIONS: This mouse model closely mimics clinical colon anastomotic leakage in humans. The model is of high clinical relevance, since anastomotic leakage has a similar cause, incidence and manifestations in humans.
KW - Analgesics, Opioid
KW - Anastomotic Leak
KW - Animals
KW - Buprenorphine
KW - Colonic Diseases
KW - Disease Models, Animal
KW - Male
KW - Mice
KW - Mice, Inbred BALB C
KW - Pain, Postoperative
KW - Pilot Projects
KW - Polyglactin 910
KW - Polypropylenes
KW - Sutures
U2 - 10.1007/s00595-013-0632-3
DO - 10.1007/s00595-013-0632-3
M3 - Journal article
C2 - 23748879
VL - 44
SP - 933
EP - 939
JO - Surgery Today
JF - Surgery Today
SN - 0941-1291
IS - 5
ER -
ID: 138429966