Colchicine treatment impairs skeletal muscle mitochondrial function and insulin sensitivity in an age-specific manner
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Colchicine treatment impairs skeletal muscle mitochondrial function and insulin sensitivity in an age-specific manner. / Buch, Bjørg Thiellesen; Halling, Jens Frey; Ringholm, Stine; Gudiksen, Anders; Kjøbsted, Rasmus; Olsen, Mette Algot; Wojtaszewski, Jørgen; Pilegaard, Henriette.
In: F A S E B Journal, Vol. 34, No. 6, 2020, p. 8653-8670.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Colchicine treatment impairs skeletal muscle mitochondrial function and insulin sensitivity in an age-specific manner
AU - Buch, Bjørg Thiellesen
AU - Halling, Jens Frey
AU - Ringholm, Stine
AU - Gudiksen, Anders
AU - Kjøbsted, Rasmus
AU - Olsen, Mette Algot
AU - Wojtaszewski, Jørgen
AU - Pilegaard, Henriette
N1 - © 2020 Federation of American Societies for Experimental Biology.
PY - 2020
Y1 - 2020
N2 - The aim of the study was to investigate the impact of autophagy inhibition on skeletal muscle mitochondrial function and glucose homeostasis in young and aged mice. The transcriptional co-activator PGC-1α regulates muscle oxidative phenotype which has been shown to be linked with basal autophagic capacity. Therefore, young and aged inducible muscle-specific PGC-1α knockout (iMKO) mice and littermate lox/lox controls were used in three separate experiments performed after either saline or colchicine injections on two consecutive days: (1) Euthanization in the basal state obtaining skeletal muscle for mitochondrial respirometry, (2) whole body glucose tolerance test, and (3) in vivo insulin-stimulated 2-deoxyglucose (2-DG) uptake into skeletal muscle. Muscle PGC-1α was not required for maintaining basal autophagy flux, regardless of age. Colchicine-induced inhibition of autophagy was associated with impairments of skeletal muscle mitochondrial function, including reduced ADP sensitivity and altered mitochondrial redox balance in both young and aged mice. Colchicine treatment reduced the glucose tolerance in aged, but not young mice, and similarly in iMKO and lox/lox mice. Colchicine reduced insulin-stimulated 2-DG uptake in soleus muscle in aged mice, independently of PGC-1α, and without affecting insulin-regulated phosphorylation of proximal or distal mediators of insulin signaling. In conclusion, the results indicate that autophagy regulates the mitochondrial ADP sensitivity and redox balance as well as whole body glucose tolerance and skeletal muscle insulin sensitivity in aged mice, with no additional effects of inducible PGC-1α deletion.
AB - The aim of the study was to investigate the impact of autophagy inhibition on skeletal muscle mitochondrial function and glucose homeostasis in young and aged mice. The transcriptional co-activator PGC-1α regulates muscle oxidative phenotype which has been shown to be linked with basal autophagic capacity. Therefore, young and aged inducible muscle-specific PGC-1α knockout (iMKO) mice and littermate lox/lox controls were used in three separate experiments performed after either saline or colchicine injections on two consecutive days: (1) Euthanization in the basal state obtaining skeletal muscle for mitochondrial respirometry, (2) whole body glucose tolerance test, and (3) in vivo insulin-stimulated 2-deoxyglucose (2-DG) uptake into skeletal muscle. Muscle PGC-1α was not required for maintaining basal autophagy flux, regardless of age. Colchicine-induced inhibition of autophagy was associated with impairments of skeletal muscle mitochondrial function, including reduced ADP sensitivity and altered mitochondrial redox balance in both young and aged mice. Colchicine treatment reduced the glucose tolerance in aged, but not young mice, and similarly in iMKO and lox/lox mice. Colchicine reduced insulin-stimulated 2-DG uptake in soleus muscle in aged mice, independently of PGC-1α, and without affecting insulin-regulated phosphorylation of proximal or distal mediators of insulin signaling. In conclusion, the results indicate that autophagy regulates the mitochondrial ADP sensitivity and redox balance as well as whole body glucose tolerance and skeletal muscle insulin sensitivity in aged mice, with no additional effects of inducible PGC-1α deletion.
KW - Faculty of Science
KW - ADP sensitivity
KW - Aging
KW - Autophagy
KW - ROS
U2 - 10.1096/fj.201903113RR
DO - 10.1096/fj.201903113RR
M3 - Journal article
C2 - 32372536
VL - 34
SP - 8653
EP - 8670
JO - F A S E B Journal
JF - F A S E B Journal
SN - 0892-6638
IS - 6
ER -
ID: 241044181