Cloning of the pig aminopeptidase N gene. Identification of possible regulatory elements and the exon distribution in relation to the membrane-spanning region.
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Cloning of the pig aminopeptidase N gene. Identification of possible regulatory elements and the exon distribution in relation to the membrane-spanning region. / Sjöström, H; Norén, O; Olsen, Jørgen.
In: FEBS Letters, Vol. 251, No. 1-2, 1989, p. 275-81.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cloning of the pig aminopeptidase N gene. Identification of possible regulatory elements and the exon distribution in relation to the membrane-spanning region.
AU - Sjöström, H
AU - Norén, O
AU - Olsen, Jørgen
N1 - Keywords: Alkaline Phosphatase; Amino Acid Sequence; Aminopeptidases; Animals; Antigens, CD; Antigens, CD13; Antigens, Differentiation, Myelomonocytic; Aspergillus Nuclease S1; Bacteriophage lambda; Base Sequence; Cell Membrane; Cloning, Molecular; DNA; DNA Restriction Enzymes; Endonucleases; Exons; Humans; Introns; Molecular Sequence Data; Nucleic Acid Hybridization; Promoter Regions (Genetics); RNA, Messenger; Regulatory Sequences, Nucleic Acid; Sequence Homology, Nucleic Acid; Swine; Transcription, Genetic; alpha 1-Antitrypsin
PY - 1989
Y1 - 1989
N2 - We have isolated four lambda-phages covering the complete pig aminopeptidase N/CD13 gene. The sequence of 2.85 kbp encompasses 1.18 kbp of the 5' upstream region and 1.67 kbp of the structural gene. In the promoter region we find a TATA box and potential binding sites for CTF-1/NF-1 and AP-2. By sequence comparisons we have found three domains showing similarity to promoter regions of the genes encoding human alpha 1-antitrypsin and human intestinal alkaline phosphatase. The gene sequence includes the first three exons and two introns. It shows that a single exon encodes the cytoplasmic tail, the membrane anchor and the junctional peptide.
AB - We have isolated four lambda-phages covering the complete pig aminopeptidase N/CD13 gene. The sequence of 2.85 kbp encompasses 1.18 kbp of the 5' upstream region and 1.67 kbp of the structural gene. In the promoter region we find a TATA box and potential binding sites for CTF-1/NF-1 and AP-2. By sequence comparisons we have found three domains showing similarity to promoter regions of the genes encoding human alpha 1-antitrypsin and human intestinal alkaline phosphatase. The gene sequence includes the first three exons and two introns. It shows that a single exon encodes the cytoplasmic tail, the membrane anchor and the junctional peptide.
M3 - Journal article
C2 - 2568950
VL - 251
SP - 275
EP - 281
JO - F E B S Letters
JF - F E B S Letters
SN - 0014-5793
IS - 1-2
ER -
ID: 6586522