Cloning by cuttings in Nordmann fir, Abies nordmanniana: hormonal characteristics in relation crown position, rooting competence, and orthotropism as ramets
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Cloning by cuttings in Nordmann fir, Abies nordmanniana : hormonal characteristics in relation crown position, rooting competence, and orthotropism as ramets. / Rasmussen, Hanne N.; Jensen, Martin; Frampton, John; Hansen-Møller, Jens; Nielsen, Ulrik Bräuner.
In: New Forests, Vol. 51, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cloning by cuttings in Nordmann fir, Abies nordmanniana
T2 - hormonal characteristics in relation crown position, rooting competence, and orthotropism as ramets
AU - Rasmussen, Hanne N.
AU - Jensen, Martin
AU - Frampton, John
AU - Hansen-Møller, Jens
AU - Nielsen, Ulrik Bräuner
PY - 2020
Y1 - 2020
N2 - Conifer tree species belonging to Abies are notoriously difficult to propagate vegetatively due to aging of material, slow rooting, and persistent plagiotropism in rooted cuttings. We produced orthotropic shoots by reiteration from strongly pruned trees (5–6 years and 12–14 years, respectively), and harvested cuttings in summer. By HPLC–MS we analyzed the stem base of each cutting for initial hormonal status, and used state-of-the art techniques for a lengthy rooting procedure. Rooting efficiency and orthotropism of the resulting ramets were recorded. Hormonal content varied significantly among cuttings types with differing positional origins on the mother tree. High rooting percentages were obtained in all types; a low original position on the main stem gave slightly better rooting but orthotropism in the ramets was slightly less frequent. Striking differences were found in cytokinin profiles between cuttings from young and mature trees. This might explain a generally lower rooting success in the latter; neither auxin levels nor the auxin: active cytokinin ratio showed any correlation with rooting capacity. Cuttings from mature trees were also less likely to remain orthotropic after rooting. Cuttings that eventually developed into orthotropic ramets were characterized by low ABA, and high levels in a range of cytokinins. The study suggests that active: conjugated cytokinins may be related to the maturation process in trees and that cytokinin content may also relate to shoot dimorphism. These results contribute to a protocol for propagation of orthotropic ramets in a Massart model tree species with strong plagiotropism.
AB - Conifer tree species belonging to Abies are notoriously difficult to propagate vegetatively due to aging of material, slow rooting, and persistent plagiotropism in rooted cuttings. We produced orthotropic shoots by reiteration from strongly pruned trees (5–6 years and 12–14 years, respectively), and harvested cuttings in summer. By HPLC–MS we analyzed the stem base of each cutting for initial hormonal status, and used state-of-the art techniques for a lengthy rooting procedure. Rooting efficiency and orthotropism of the resulting ramets were recorded. Hormonal content varied significantly among cuttings types with differing positional origins on the mother tree. High rooting percentages were obtained in all types; a low original position on the main stem gave slightly better rooting but orthotropism in the ramets was slightly less frequent. Striking differences were found in cytokinin profiles between cuttings from young and mature trees. This might explain a generally lower rooting success in the latter; neither auxin levels nor the auxin: active cytokinin ratio showed any correlation with rooting capacity. Cuttings from mature trees were also less likely to remain orthotropic after rooting. Cuttings that eventually developed into orthotropic ramets were characterized by low ABA, and high levels in a range of cytokinins. The study suggests that active: conjugated cytokinins may be related to the maturation process in trees and that cytokinin content may also relate to shoot dimorphism. These results contribute to a protocol for propagation of orthotropic ramets in a Massart model tree species with strong plagiotropism.
KW - Abscisic acid
KW - Adventitious rooting
KW - Aging
KW - Auxin
KW - Conifer
KW - Conjugated cytokinins
KW - Plagiotropism
KW - Topophysis
U2 - 10.1007/s11056-019-09759-0
DO - 10.1007/s11056-019-09759-0
M3 - Journal article
AN - SCOPUS:85074684700
VL - 51
JO - New Forests
JF - New Forests
SN - 0169-4286
ER -
ID: 230390241