Circular RNAs are a large class of animal RNAs with regulatory potency

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Circular RNAs are a large class of animal RNAs with regulatory potency. / Memczak, Sebastian; Jens, Marvin; Elefsinioti, Antigoni; Torti, Francesca; Krueger, Janna; Rybak, Agnieszka; Maier, Luisa; Mackowiak, Sebastian D; Gregersen, Lea H; Munschauer, Mathias; Loewer, Alexander; Ziebold, Ulrike; Landthaler, Markus; Kocks, Christine; le Noble, Ferdinand; Rajewsky, Nikolaus.

In: Nature, Vol. 495, No. 7441, 21.03.2013, p. 333-8.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Memczak, S, Jens, M, Elefsinioti, A, Torti, F, Krueger, J, Rybak, A, Maier, L, Mackowiak, SD, Gregersen, LH, Munschauer, M, Loewer, A, Ziebold, U, Landthaler, M, Kocks, C, le Noble, F & Rajewsky, N 2013, 'Circular RNAs are a large class of animal RNAs with regulatory potency', Nature, vol. 495, no. 7441, pp. 333-8. https://doi.org/10.1038/nature11928

APA

Memczak, S., Jens, M., Elefsinioti, A., Torti, F., Krueger, J., Rybak, A., Maier, L., Mackowiak, S. D., Gregersen, L. H., Munschauer, M., Loewer, A., Ziebold, U., Landthaler, M., Kocks, C., le Noble, F., & Rajewsky, N. (2013). Circular RNAs are a large class of animal RNAs with regulatory potency. Nature, 495(7441), 333-8. https://doi.org/10.1038/nature11928

Vancouver

Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A et al. Circular RNAs are a large class of animal RNAs with regulatory potency. Nature. 2013 Mar 21;495(7441):333-8. https://doi.org/10.1038/nature11928

Author

Memczak, Sebastian ; Jens, Marvin ; Elefsinioti, Antigoni ; Torti, Francesca ; Krueger, Janna ; Rybak, Agnieszka ; Maier, Luisa ; Mackowiak, Sebastian D ; Gregersen, Lea H ; Munschauer, Mathias ; Loewer, Alexander ; Ziebold, Ulrike ; Landthaler, Markus ; Kocks, Christine ; le Noble, Ferdinand ; Rajewsky, Nikolaus. / Circular RNAs are a large class of animal RNAs with regulatory potency. In: Nature. 2013 ; Vol. 495, No. 7441. pp. 333-8.

Bibtex

@article{c207a402c1bd4977a75366083cb5245d,
title = "Circular RNAs are a large class of animal RNAs with regulatory potency",
abstract = "Circular RNAs (circRNAs) in animals are an enigmatic class of RNA with unknown function. To explore circRNAs systematically, we sequenced and computationally analysed human, mouse and nematode RNA. We detected thousands of well-expressed, stable circRNAs, often showing tissue/developmental-stage-specific expression. Sequence analysis indicated important regulatory functions for circRNAs. We found that a human circRNA, antisense to the cerebellar degeneration-related protein 1 transcript (CDR1as), is densely bound by microRNA (miRNA) effector complexes and harbours 63 conserved binding sites for the ancient miRNA miR-7. Further analyses indicated that CDR1as functions to bind miR-7 in neuronal tissues. Human CDR1as expression in zebrafish impaired midbrain development, similar to knocking down miR-7, suggesting that CDR1as is a miRNA antagonist with a miRNA-binding capacity ten times higher than any other known transcript. Together, our data provide evidence that circRNAs form a large class of post-transcriptional regulators. Numerous circRNAs form by head-to-tail splicing of exons, suggesting previously unrecognized regulatory potential of coding sequences.",
keywords = "Animals, Autoantigens/genetics, Binding Sites, Brain/metabolism, Caenorhabditis elegans/genetics, Cell Line, Conserved Sequence, Female, Gene Expression Regulation, HEK293 Cells, Humans, Male, Mice, MicroRNAs/genetics, Nerve Tissue Proteins/genetics, RNA/genetics, RNA, Circular, Zebrafish/embryology",
author = "Sebastian Memczak and Marvin Jens and Antigoni Elefsinioti and Francesca Torti and Janna Krueger and Agnieszka Rybak and Luisa Maier and Mackowiak, {Sebastian D} and Gregersen, {Lea H} and Mathias Munschauer and Alexander Loewer and Ulrike Ziebold and Markus Landthaler and Christine Kocks and {le Noble}, Ferdinand and Nikolaus Rajewsky",
year = "2013",
month = mar,
day = "21",
doi = "10.1038/nature11928",
language = "English",
volume = "495",
pages = "333--8",
journal = "Nature",
issn = "0028-0836",
publisher = "nature publishing group",
number = "7441",

}

RIS

TY - JOUR

T1 - Circular RNAs are a large class of animal RNAs with regulatory potency

AU - Memczak, Sebastian

AU - Jens, Marvin

AU - Elefsinioti, Antigoni

AU - Torti, Francesca

AU - Krueger, Janna

AU - Rybak, Agnieszka

AU - Maier, Luisa

AU - Mackowiak, Sebastian D

AU - Gregersen, Lea H

AU - Munschauer, Mathias

AU - Loewer, Alexander

AU - Ziebold, Ulrike

AU - Landthaler, Markus

AU - Kocks, Christine

AU - le Noble, Ferdinand

AU - Rajewsky, Nikolaus

PY - 2013/3/21

Y1 - 2013/3/21

N2 - Circular RNAs (circRNAs) in animals are an enigmatic class of RNA with unknown function. To explore circRNAs systematically, we sequenced and computationally analysed human, mouse and nematode RNA. We detected thousands of well-expressed, stable circRNAs, often showing tissue/developmental-stage-specific expression. Sequence analysis indicated important regulatory functions for circRNAs. We found that a human circRNA, antisense to the cerebellar degeneration-related protein 1 transcript (CDR1as), is densely bound by microRNA (miRNA) effector complexes and harbours 63 conserved binding sites for the ancient miRNA miR-7. Further analyses indicated that CDR1as functions to bind miR-7 in neuronal tissues. Human CDR1as expression in zebrafish impaired midbrain development, similar to knocking down miR-7, suggesting that CDR1as is a miRNA antagonist with a miRNA-binding capacity ten times higher than any other known transcript. Together, our data provide evidence that circRNAs form a large class of post-transcriptional regulators. Numerous circRNAs form by head-to-tail splicing of exons, suggesting previously unrecognized regulatory potential of coding sequences.

AB - Circular RNAs (circRNAs) in animals are an enigmatic class of RNA with unknown function. To explore circRNAs systematically, we sequenced and computationally analysed human, mouse and nematode RNA. We detected thousands of well-expressed, stable circRNAs, often showing tissue/developmental-stage-specific expression. Sequence analysis indicated important regulatory functions for circRNAs. We found that a human circRNA, antisense to the cerebellar degeneration-related protein 1 transcript (CDR1as), is densely bound by microRNA (miRNA) effector complexes and harbours 63 conserved binding sites for the ancient miRNA miR-7. Further analyses indicated that CDR1as functions to bind miR-7 in neuronal tissues. Human CDR1as expression in zebrafish impaired midbrain development, similar to knocking down miR-7, suggesting that CDR1as is a miRNA antagonist with a miRNA-binding capacity ten times higher than any other known transcript. Together, our data provide evidence that circRNAs form a large class of post-transcriptional regulators. Numerous circRNAs form by head-to-tail splicing of exons, suggesting previously unrecognized regulatory potential of coding sequences.

KW - Animals

KW - Autoantigens/genetics

KW - Binding Sites

KW - Brain/metabolism

KW - Caenorhabditis elegans/genetics

KW - Cell Line

KW - Conserved Sequence

KW - Female

KW - Gene Expression Regulation

KW - HEK293 Cells

KW - Humans

KW - Male

KW - Mice

KW - MicroRNAs/genetics

KW - Nerve Tissue Proteins/genetics

KW - RNA/genetics

KW - RNA, Circular

KW - Zebrafish/embryology

U2 - 10.1038/nature11928

DO - 10.1038/nature11928

M3 - Journal article

C2 - 23446348

VL - 495

SP - 333

EP - 338

JO - Nature

JF - Nature

SN - 0028-0836

IS - 7441

ER -

ID: 328236755