TY - JOUR

T1 - Chromatin elongation factors

AU - Svejstrup, Jesper Q.

N1 - Funding Information: Work in my laboratory is supported by Cancer Research UK and a grant from the European Community (HPRN-CT-2000-00087).

PY - 2002/4/1

Y1 - 2002/4/1

N2 - As RNA polymerase II leaves a gene promoter to transcribe the coding region, it faces a major obstacle - nucleosomes tightly wrapped into chromatin. Mechanisms to deal with this obstacle clearly exist in cells, as transcription through chromatin is very efficient in vivo, whereas nucleosomal templates pose a considerable problem for polymerase progression in reconstituted in vitro systems. Advances in our understanding of transcriptional elongation through chromatin have been made possible recently by the identification of several accessory factors that assist polymerase in the process. Insights into the function of these factors have been gained by a combination of yeast genetics and biochemical studies in mammalian systems.

AB - As RNA polymerase II leaves a gene promoter to transcribe the coding region, it faces a major obstacle - nucleosomes tightly wrapped into chromatin. Mechanisms to deal with this obstacle clearly exist in cells, as transcription through chromatin is very efficient in vivo, whereas nucleosomal templates pose a considerable problem for polymerase progression in reconstituted in vitro systems. Advances in our understanding of transcriptional elongation through chromatin have been made possible recently by the identification of several accessory factors that assist polymerase in the process. Insights into the function of these factors have been gained by a combination of yeast genetics and biochemical studies in mammalian systems.

U2 - 10.1016/S0959-437X(02)00281-2

DO - 10.1016/S0959-437X(02)00281-2

M3 - Review

C2 - 11893488

AN - SCOPUS:0036531899

VL - 12

SP - 156

EP - 161

JO - Current Opinion in Genetics & Development

JF - Current Opinion in Genetics & Development

SN - 0959-437X

IS - 2

ER -