β-Carotene does not change markers of enzymatic and nonenzymatic antioxidant activity in human blood
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β-Carotene does not change markers of enzymatic and nonenzymatic antioxidant activity in human blood. / Castenmiller, Jacqueline J M; Lauridsen, Søren T; Dragsted, Lars Ove; van Het Hof, Karin; Linssen, Jozef P H; West, Clive E.
In: Journal of Nutrition, Vol. 129, No. 12, 1999, p. 2162-2169.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - β-Carotene does not change markers of enzymatic and nonenzymatic antioxidant activity in human blood
AU - Castenmiller, Jacqueline J M
AU - Lauridsen, Søren T
AU - Dragsted, Lars Ove
AU - van Het Hof, Karin
AU - Linssen, Jozef P H
AU - West, Clive E
N1 - (Ekstern)
PY - 1999
Y1 - 1999
N2 - In vitamin A-replete populations, increased concentrations of serum carotenoids have been associated with a decreased risk of degenerative diseases. The mechanism of action of carotenoids in determining antioxidant activity is largely unknown. The aim of the study was to examine the effect of carotenoid supplementation and spinach intake on erythrocyte enzyme antioxidant activities, serum or plasma nonenzymatic antioxidant concentrations, and concentrations of oxidatively damaged amino acids in plasma. Subjects received for 3 wk a basic diet (n = 10), a basic diet with a carotenoid supplement (n = 12) or with a spinach product (n 12 per group), i.e., whole-leaf, minced, liquefied or liquefied spinach plus added dietary fiber. After 3 wk of dietary intervention, changes in serum or plasma concentrations of ascorbic acid, α-tocopherol, FRAP (ferric reducing ability of plasma) and uric acid and erythrocyte enzyme activities were assessed, and differences among experimental groups were tested. Consumption of spinach resulted in greater (P < 0.01) erythrocyte glutathione reductase activity and lower (P < 0.05) erythrocyte catalase activity and serum α-tocopherol concentration compared with the control group. Consumption of the carotenoid supplement led to lower α-tocopherol responses (P = 0.02) compared with the basic diet only. Our data suggest that the short-term changes in erythrocyte glutathione reductase activity and serum α-tocopherol concentration can be attributed to an increased carotenoid (lutein and zeaxanthin) intake, but β- carotene is unlikely to be a causative factor. Lower erythrocyte catalase activity after intervention with spinach products may be related to other constituents in spinach such as flavonoids.
AB - In vitamin A-replete populations, increased concentrations of serum carotenoids have been associated with a decreased risk of degenerative diseases. The mechanism of action of carotenoids in determining antioxidant activity is largely unknown. The aim of the study was to examine the effect of carotenoid supplementation and spinach intake on erythrocyte enzyme antioxidant activities, serum or plasma nonenzymatic antioxidant concentrations, and concentrations of oxidatively damaged amino acids in plasma. Subjects received for 3 wk a basic diet (n = 10), a basic diet with a carotenoid supplement (n = 12) or with a spinach product (n 12 per group), i.e., whole-leaf, minced, liquefied or liquefied spinach plus added dietary fiber. After 3 wk of dietary intervention, changes in serum or plasma concentrations of ascorbic acid, α-tocopherol, FRAP (ferric reducing ability of plasma) and uric acid and erythrocyte enzyme activities were assessed, and differences among experimental groups were tested. Consumption of spinach resulted in greater (P < 0.01) erythrocyte glutathione reductase activity and lower (P < 0.05) erythrocyte catalase activity and serum α-tocopherol concentration compared with the control group. Consumption of the carotenoid supplement led to lower α-tocopherol responses (P = 0.02) compared with the basic diet only. Our data suggest that the short-term changes in erythrocyte glutathione reductase activity and serum α-tocopherol concentration can be attributed to an increased carotenoid (lutein and zeaxanthin) intake, but β- carotene is unlikely to be a causative factor. Lower erythrocyte catalase activity after intervention with spinach products may be related to other constituents in spinach such as flavonoids.
KW - Antioxidants
KW - Carotenoids
KW - Humans
KW - Spinach
KW - α-tocopherol
UR - http://www.scopus.com/inward/record.url?scp=0032701776&partnerID=8YFLogxK
U2 - 10.1093/jn/129.12.2162
DO - 10.1093/jn/129.12.2162
M3 - Journal article
C2 - 10573544
AN - SCOPUS:0032701776
VL - 129
SP - 2162
EP - 2169
JO - Journal of Nutrition
JF - Journal of Nutrition
SN - 0022-3166
IS - 12
ER -
ID: 254729998