Infections are the most common cause of bovine abortion. Here we report recent diagnostic findings in bovine abortion material from Denmark, a country with a large dairy sector and high animal health standards. This study was conducted in order to gain in-depth knowledge on infectious causes of abortions i.e. to identify and localize infectious agents in placental and foetal tissues. The cultivation-independent methods Fluorescence in situ hybridization (FISH) and second generation sequencing were applied additionally to routine histopathology and bacterial cultivation