Biogenesis of the mitochondrial DNA inheritance machinery in the mitochondrial outer membrane of Trypanosoma brucei
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Biogenesis of the mitochondrial DNA inheritance machinery in the mitochondrial outer membrane of Trypanosoma brucei. / Käser, Sandro; Willemin, Mathilde; Schnarwiler, Felix; Schimanski, Bernd; Poveda-Huertes, Daniel; Oeljeklaus, Silke; Haenni, Beat; Zuber, Benoît; Warscheid, Bettina; Meisinger, Chris; Schneider, André.
In: PLoS Pathogens, Vol. 13, No. 12, 12.2017, p. e1006808.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Biogenesis of the mitochondrial DNA inheritance machinery in the mitochondrial outer membrane of Trypanosoma brucei
AU - Käser, Sandro
AU - Willemin, Mathilde
AU - Schnarwiler, Felix
AU - Schimanski, Bernd
AU - Poveda-Huertes, Daniel
AU - Oeljeklaus, Silke
AU - Haenni, Beat
AU - Zuber, Benoît
AU - Warscheid, Bettina
AU - Meisinger, Chris
AU - Schneider, André
PY - 2017/12
Y1 - 2017/12
N2 - Mitochondria cannot form de novo but require mechanisms that mediate their inheritance to daughter cells. The parasitic protozoan Trypanosoma brucei has a single mitochondrion with a single-unit genome that is physically connected across the two mitochondrial membranes with the basal body of the flagellum. This connection, termed the tripartite attachment complex (TAC), is essential for the segregation of the replicated mitochondrial genomes prior to cytokinesis. Here we identify a protein complex consisting of three integral mitochondrial outer membrane proteins-TAC60, TAC42 and TAC40-which are essential subunits of the TAC. TAC60 contains separable mitochondrial import and TAC-sorting signals and its biogenesis depends on the main outer membrane protein translocase. TAC40 is a member of the mitochondrial porin family, whereas TAC42 represents a novel class of mitochondrial outer membrane β-barrel proteins. Consequently TAC40 and TAC42 contain C-terminal β-signals. Thus in trypanosomes the highly conserved β-barrel protein assembly machinery plays a major role in the biogenesis of its unique mitochondrial genome segregation system.
AB - Mitochondria cannot form de novo but require mechanisms that mediate their inheritance to daughter cells. The parasitic protozoan Trypanosoma brucei has a single mitochondrion with a single-unit genome that is physically connected across the two mitochondrial membranes with the basal body of the flagellum. This connection, termed the tripartite attachment complex (TAC), is essential for the segregation of the replicated mitochondrial genomes prior to cytokinesis. Here we identify a protein complex consisting of three integral mitochondrial outer membrane proteins-TAC60, TAC42 and TAC40-which are essential subunits of the TAC. TAC60 contains separable mitochondrial import and TAC-sorting signals and its biogenesis depends on the main outer membrane protein translocase. TAC40 is a member of the mitochondrial porin family, whereas TAC42 represents a novel class of mitochondrial outer membrane β-barrel proteins. Consequently TAC40 and TAC42 contain C-terminal β-signals. Thus in trypanosomes the highly conserved β-barrel protein assembly machinery plays a major role in the biogenesis of its unique mitochondrial genome segregation system.
KW - Animals
KW - DNA, Kinetoplast/biosynthesis
KW - DNA, Mitochondrial/biosynthesis
KW - Genome, Mitochondrial
KW - Genome, Protozoan
KW - Humans
KW - Mitochondrial Dynamics
KW - Mitochondrial Membranes/metabolism
KW - Multiprotein Complexes/chemistry
KW - Protein Sorting Signals/genetics
KW - Protozoan Proteins/chemistry
KW - Trypanosoma brucei brucei/genetics
U2 - 10.1371/journal.ppat.1006808
DO - 10.1371/journal.ppat.1006808
M3 - Journal article
C2 - 29287109
VL - 13
SP - e1006808
JO - P L o S Pathogens (Online)
JF - P L o S Pathogens (Online)
SN - 1553-7374
IS - 12
ER -
ID: 391635475