Assays for urinary biomarkers of oxidatively damaged nucleic acids
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Assays for urinary biomarkers of oxidatively damaged nucleic acids. / Weimann, Allan; Broedbaek, Kasper; Henriksen, Trine; Stovgaard, Elisabeth Ida Specht; Poulsen, Henrik Enghusen.
In: Free Radical Research, Vol. 46, No. 4, 2012, p. 531-40.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Assays for urinary biomarkers of oxidatively damaged nucleic acids
AU - Weimann, Allan
AU - Broedbaek, Kasper
AU - Henriksen, Trine
AU - Stovgaard, Elisabeth Ida Specht
AU - Poulsen, Henrik Enghusen
PY - 2012
Y1 - 2012
N2 - Abstract The analysis of oxidized nucleic acid metabolites can be performed by a variety of methodologies: liquid chromatography coupled with electrochemical or mass-spectrometry detection, gas chromatography coupled with mass spectrometry, capillary electrophoresis and ELISA (Enzyme-linked immunosorbent assay). The major analytical challenge is specificity. The best combination of selectivity and speed of analysis can be obtained by liquid chromatography coupled with tandem mass spectrometric detection. This, however, is also the most demanding technique with regard to price, complexity and skills requirement. The available ELISA methods present considerable specificity problems and cannot be recommended at present. The oxidized nucleic acid metabolites in urine are assumed to originate from the DNA and RNA. However, direct evidence is not available. A possible contribution from the nucleotide pools is most probably minimal, if existing. Recent investigation on RNA oxidation has shown conditions where RNA oxidation but not DNA oxidation is prominent, and while investigation on DNA is of huge interest, RNA oxidation may be overlooked. The methods for analyzing oxidized deoxynucleosides can easily be expanded to analyze the oxidized ribonucleosides. The urinary measurement of oxidized nucleic acid metabolites provides a non-invasive measurement of oxidative stress to DNA and RNA.
AB - Abstract The analysis of oxidized nucleic acid metabolites can be performed by a variety of methodologies: liquid chromatography coupled with electrochemical or mass-spectrometry detection, gas chromatography coupled with mass spectrometry, capillary electrophoresis and ELISA (Enzyme-linked immunosorbent assay). The major analytical challenge is specificity. The best combination of selectivity and speed of analysis can be obtained by liquid chromatography coupled with tandem mass spectrometric detection. This, however, is also the most demanding technique with regard to price, complexity and skills requirement. The available ELISA methods present considerable specificity problems and cannot be recommended at present. The oxidized nucleic acid metabolites in urine are assumed to originate from the DNA and RNA. However, direct evidence is not available. A possible contribution from the nucleotide pools is most probably minimal, if existing. Recent investigation on RNA oxidation has shown conditions where RNA oxidation but not DNA oxidation is prominent, and while investigation on DNA is of huge interest, RNA oxidation may be overlooked. The methods for analyzing oxidized deoxynucleosides can easily be expanded to analyze the oxidized ribonucleosides. The urinary measurement of oxidized nucleic acid metabolites provides a non-invasive measurement of oxidative stress to DNA and RNA.
U2 - 10.3109/10715762.2011.647693
DO - 10.3109/10715762.2011.647693
M3 - Journal article
C2 - 22352957
VL - 46
SP - 531
EP - 540
JO - Free Radical Research
JF - Free Radical Research
SN - 1071-5762
IS - 4
ER -
ID: 40189764