Assays for urinary biomarkers of oxidatively damaged nucleic acids

Research output: Contribution to journalJournal articleResearchpeer-review

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Assays for urinary biomarkers of oxidatively damaged nucleic acids. / Weimann, Allan; Broedbaek, Kasper; Henriksen, Trine; Stovgaard, Elisabeth Ida Specht; Poulsen, Henrik Enghusen.

In: Free Radical Research, Vol. 46, No. 4, 2012, p. 531-40.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Weimann, A, Broedbaek, K, Henriksen, T, Stovgaard, EIS & Poulsen, HE 2012, 'Assays for urinary biomarkers of oxidatively damaged nucleic acids', Free Radical Research, vol. 46, no. 4, pp. 531-40. https://doi.org/10.3109/10715762.2011.647693

APA

Weimann, A., Broedbaek, K., Henriksen, T., Stovgaard, E. I. S., & Poulsen, H. E. (2012). Assays for urinary biomarkers of oxidatively damaged nucleic acids. Free Radical Research, 46(4), 531-40. https://doi.org/10.3109/10715762.2011.647693

Vancouver

Weimann A, Broedbaek K, Henriksen T, Stovgaard EIS, Poulsen HE. Assays for urinary biomarkers of oxidatively damaged nucleic acids. Free Radical Research. 2012;46(4):531-40. https://doi.org/10.3109/10715762.2011.647693

Author

Weimann, Allan ; Broedbaek, Kasper ; Henriksen, Trine ; Stovgaard, Elisabeth Ida Specht ; Poulsen, Henrik Enghusen. / Assays for urinary biomarkers of oxidatively damaged nucleic acids. In: Free Radical Research. 2012 ; Vol. 46, No. 4. pp. 531-40.

Bibtex

@article{cdc06cffbaf842ddb9df55f152e79469,
title = "Assays for urinary biomarkers of oxidatively damaged nucleic acids",
abstract = "Abstract The analysis of oxidized nucleic acid metabolites can be performed by a variety of methodologies: liquid chromatography coupled with electrochemical or mass-spectrometry detection, gas chromatography coupled with mass spectrometry, capillary electrophoresis and ELISA (Enzyme-linked immunosorbent assay). The major analytical challenge is specificity. The best combination of selectivity and speed of analysis can be obtained by liquid chromatography coupled with tandem mass spectrometric detection. This, however, is also the most demanding technique with regard to price, complexity and skills requirement. The available ELISA methods present considerable specificity problems and cannot be recommended at present. The oxidized nucleic acid metabolites in urine are assumed to originate from the DNA and RNA. However, direct evidence is not available. A possible contribution from the nucleotide pools is most probably minimal, if existing. Recent investigation on RNA oxidation has shown conditions where RNA oxidation but not DNA oxidation is prominent, and while investigation on DNA is of huge interest, RNA oxidation may be overlooked. The methods for analyzing oxidized deoxynucleosides can easily be expanded to analyze the oxidized ribonucleosides. The urinary measurement of oxidized nucleic acid metabolites provides a non-invasive measurement of oxidative stress to DNA and RNA.",
author = "Allan Weimann and Kasper Broedbaek and Trine Henriksen and Stovgaard, {Elisabeth Ida Specht} and Poulsen, {Henrik Enghusen}",
year = "2012",
doi = "10.3109/10715762.2011.647693",
language = "English",
volume = "46",
pages = "531--40",
journal = "Free Radical Research",
issn = "1071-5762",
publisher = "Taylor & Francis",
number = "4",

}

RIS

TY - JOUR

T1 - Assays for urinary biomarkers of oxidatively damaged nucleic acids

AU - Weimann, Allan

AU - Broedbaek, Kasper

AU - Henriksen, Trine

AU - Stovgaard, Elisabeth Ida Specht

AU - Poulsen, Henrik Enghusen

PY - 2012

Y1 - 2012

N2 - Abstract The analysis of oxidized nucleic acid metabolites can be performed by a variety of methodologies: liquid chromatography coupled with electrochemical or mass-spectrometry detection, gas chromatography coupled with mass spectrometry, capillary electrophoresis and ELISA (Enzyme-linked immunosorbent assay). The major analytical challenge is specificity. The best combination of selectivity and speed of analysis can be obtained by liquid chromatography coupled with tandem mass spectrometric detection. This, however, is also the most demanding technique with regard to price, complexity and skills requirement. The available ELISA methods present considerable specificity problems and cannot be recommended at present. The oxidized nucleic acid metabolites in urine are assumed to originate from the DNA and RNA. However, direct evidence is not available. A possible contribution from the nucleotide pools is most probably minimal, if existing. Recent investigation on RNA oxidation has shown conditions where RNA oxidation but not DNA oxidation is prominent, and while investigation on DNA is of huge interest, RNA oxidation may be overlooked. The methods for analyzing oxidized deoxynucleosides can easily be expanded to analyze the oxidized ribonucleosides. The urinary measurement of oxidized nucleic acid metabolites provides a non-invasive measurement of oxidative stress to DNA and RNA.

AB - Abstract The analysis of oxidized nucleic acid metabolites can be performed by a variety of methodologies: liquid chromatography coupled with electrochemical or mass-spectrometry detection, gas chromatography coupled with mass spectrometry, capillary electrophoresis and ELISA (Enzyme-linked immunosorbent assay). The major analytical challenge is specificity. The best combination of selectivity and speed of analysis can be obtained by liquid chromatography coupled with tandem mass spectrometric detection. This, however, is also the most demanding technique with regard to price, complexity and skills requirement. The available ELISA methods present considerable specificity problems and cannot be recommended at present. The oxidized nucleic acid metabolites in urine are assumed to originate from the DNA and RNA. However, direct evidence is not available. A possible contribution from the nucleotide pools is most probably minimal, if existing. Recent investigation on RNA oxidation has shown conditions where RNA oxidation but not DNA oxidation is prominent, and while investigation on DNA is of huge interest, RNA oxidation may be overlooked. The methods for analyzing oxidized deoxynucleosides can easily be expanded to analyze the oxidized ribonucleosides. The urinary measurement of oxidized nucleic acid metabolites provides a non-invasive measurement of oxidative stress to DNA and RNA.

U2 - 10.3109/10715762.2011.647693

DO - 10.3109/10715762.2011.647693

M3 - Journal article

C2 - 22352957

VL - 46

SP - 531

EP - 540

JO - Free Radical Research

JF - Free Radical Research

SN - 1071-5762

IS - 4

ER -

ID: 40189764