Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates
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Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates. / Grimmelikhuijzen, C J; Ebbesen, Ditte Graff.
In: Peptides, Vol. 6 Suppl 3, 01.01.1985, p. 477-83.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates
AU - Grimmelikhuijzen, C J
AU - Ebbesen, Ditte Graff
PY - 1985/1/1
Y1 - 1985/1/1
N2 - By using immunocytochemistry and radioimmunoassays, several substances resembling vertebrate or invertebrate neuropeptides have been found in the nervous systems of coelenterates. The most abundant neuropeptides were those related to the molluscan neuropeptide Phe-Met-Arg-Phe-amide (FMRFamide). Of antisera against different fragments of FMRFamide, those against RFamide were superior in recognizing the coelenterate peptide. Incubation of whole mounts with these RFamide antisera visualized the coelenterate nervous system in such a detail as has previously not been possible. By using a radioimmunoassay with a RFamide antiserum and [J-125]-YFMRFamide as tracer, the RFamide-like peptide from sea anemones was isolated. After cation-exchange chromatography, gelfiltration and HPLC, this peptide was obtained in a pure form.
AB - By using immunocytochemistry and radioimmunoassays, several substances resembling vertebrate or invertebrate neuropeptides have been found in the nervous systems of coelenterates. The most abundant neuropeptides were those related to the molluscan neuropeptide Phe-Met-Arg-Phe-amide (FMRFamide). Of antisera against different fragments of FMRFamide, those against RFamide were superior in recognizing the coelenterate peptide. Incubation of whole mounts with these RFamide antisera visualized the coelenterate nervous system in such a detail as has previously not been possible. By using a radioimmunoassay with a RFamide antiserum and [J-125]-YFMRFamide as tracer, the RFamide-like peptide from sea anemones was isolated. After cation-exchange chromatography, gelfiltration and HPLC, this peptide was obtained in a pure form.
KW - Animals
KW - Chromatography
KW - Cnidaria
KW - FMRFamide
KW - Fluorescent Antibody Technique
KW - Nerve Tissue Proteins
KW - Nervous System Physiological Phenomena
KW - Oligopeptides
KW - Radioimmunoassay
M3 - Journal article
C2 - 2870476
VL - 6 Suppl 3
SP - 477
EP - 483
JO - Peptides
JF - Peptides
SN - 0196-9781
ER -
ID: 33514620