Arachidonic acid metabolism in neutrophil granulocytes obtained from synovial fluid in rheumatoid arthritis
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Arachidonic acid metabolism in neutrophil granulocytes obtained from synovial fluid in rheumatoid arthritis. / Langholz, E; Nielsen, O H; Ahnfelt-Rønne, I; Elmgreen, J.
In: Scandinavian Journal of Rheumatology, Vol. 19, No. 6, 1990, p. 387-91.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Arachidonic acid metabolism in neutrophil granulocytes obtained from synovial fluid in rheumatoid arthritis
AU - Langholz, E
AU - Nielsen, O H
AU - Ahnfelt-Rønne, I
AU - Elmgreen, J
PY - 1990
Y1 - 1990
N2 - Circulating human neutrophil granulocytes (PMNs) from patients with rheumatoid arthritis (RA) have earlier been described to possess an enhanced capacity for production of certain 5-lipoxygenase-derived metabolites of arachidonic acid (AA), 5-hydroxyeicosatetraenoic acid (5-HETE) and leukotriene B4 (LTB4). In the present investigation the endogenous AA metabolism of synovial fluid PMNs of RA patients was studied and compared with that of the corresponding circulating PMNs. Synovial fluid PMNs prelabelled with 14C-AA released significantly less radioactivity than circulating PMNs when stimulated with calcium ionophore. Furthermore, synovial fluid PMNs produced significantly smaller amounts of both 5-HETE and LTB4 than circulating PMNs from the same patients, whereas no such difference was observed in the LTB4 catabolites or the cyclo-oxygenase products. More information dealing with the complex way in which arachidonic acid is metabolized in diseased RA joints may provide future rational approaches in the treatment of this chronic inflammatory disease.
AB - Circulating human neutrophil granulocytes (PMNs) from patients with rheumatoid arthritis (RA) have earlier been described to possess an enhanced capacity for production of certain 5-lipoxygenase-derived metabolites of arachidonic acid (AA), 5-hydroxyeicosatetraenoic acid (5-HETE) and leukotriene B4 (LTB4). In the present investigation the endogenous AA metabolism of synovial fluid PMNs of RA patients was studied and compared with that of the corresponding circulating PMNs. Synovial fluid PMNs prelabelled with 14C-AA released significantly less radioactivity than circulating PMNs when stimulated with calcium ionophore. Furthermore, synovial fluid PMNs produced significantly smaller amounts of both 5-HETE and LTB4 than circulating PMNs from the same patients, whereas no such difference was observed in the LTB4 catabolites or the cyclo-oxygenase products. More information dealing with the complex way in which arachidonic acid is metabolized in diseased RA joints may provide future rational approaches in the treatment of this chronic inflammatory disease.
KW - Adult
KW - Aged
KW - Arachidonic Acids/metabolism
KW - Arthritis, Rheumatoid/blood
KW - Female
KW - Humans
KW - Hydroxyeicosatetraenoic Acids/blood
KW - Leukotriene B4/blood
KW - Male
KW - Middle Aged
KW - Neutrophils/metabolism
KW - Synovial Fluid/cytology
M3 - Journal article
C2 - 2175453
VL - 19
SP - 387
EP - 391
JO - Scandinavian Journal of Rheumatology
JF - Scandinavian Journal of Rheumatology
SN - 0300-9742
IS - 6
ER -
ID: 218728431