Antagonism of the interleukin 4 receptor α promotes TH1-signalling among T cells from patients with atopic dermatitis after stimulation
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Antagonism of the interleukin 4 receptor α promotes TH1-signalling among T cells from patients with atopic dermatitis after stimulation. / Brøgger, Peter; Blom, Lars H; Simonsen, Stine; Thyssen, Jacob P; Skov, Lone.
In: Scandinavian Journal of Immunology, Vol. 91, No. 1, e12835, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Antagonism of the interleukin 4 receptor α promotes TH1-signalling among T cells from patients with atopic dermatitis after stimulation
AU - Brøgger, Peter
AU - Blom, Lars H
AU - Simonsen, Stine
AU - Thyssen, Jacob P
AU - Skov, Lone
N1 - © 2019 The Scandinavian Foundation for Immunology.
PY - 2020
Y1 - 2020
N2 - Atopic dermatitis (AD) is a chronic and relapsing inflammatory skin disease. Molecular characterization of AD shows an underlying inflammation with tissue infiltration of T helper (TH ) 2 cells and increased IL-4 and IL-13. The multifaceted roles of IL-4 and IL-13 in allergic disease development make IL-4Rα an attractive target for treatment strategies, and a neutralizing monoclonal antibody which antagonizes the effects of both IL-4 and IL-13 by blocking the interaction site found in the IL-4 receptor subunit α (IL-4Rα) has been successfully used to treat patients with moderate-to-severe AD. To elucidate the effects of IL-4Rα blockade on the cellular level, we used flow cytometry to examine cytokine production after antigen stimulation in human T cells from patients with AD (n = 12) and healthy controls (n = 6). The cells were stimulated with and without a neutralizing monoclonal antibody against IL-4Rα. Our results indicate that blocking IL-4Rα prohibits IL-4 signalling and IL-13 signalling and thereby TH 2 differentiation followed by an upregulation of interferon-γ-producing cells.
AB - Atopic dermatitis (AD) is a chronic and relapsing inflammatory skin disease. Molecular characterization of AD shows an underlying inflammation with tissue infiltration of T helper (TH ) 2 cells and increased IL-4 and IL-13. The multifaceted roles of IL-4 and IL-13 in allergic disease development make IL-4Rα an attractive target for treatment strategies, and a neutralizing monoclonal antibody which antagonizes the effects of both IL-4 and IL-13 by blocking the interaction site found in the IL-4 receptor subunit α (IL-4Rα) has been successfully used to treat patients with moderate-to-severe AD. To elucidate the effects of IL-4Rα blockade on the cellular level, we used flow cytometry to examine cytokine production after antigen stimulation in human T cells from patients with AD (n = 12) and healthy controls (n = 6). The cells were stimulated with and without a neutralizing monoclonal antibody against IL-4Rα. Our results indicate that blocking IL-4Rα prohibits IL-4 signalling and IL-13 signalling and thereby TH 2 differentiation followed by an upregulation of interferon-γ-producing cells.
KW - Adult
KW - Biomarkers
KW - Cytokines/metabolism
KW - Dermatitis, Atopic/immunology
KW - Female
KW - Humans
KW - Immunoglobulin E/blood
KW - Interleukin-4 Receptor alpha Subunit/antagonists & inhibitors
KW - Leukocytes, Mononuclear/immunology
KW - Male
KW - Middle Aged
KW - Signal Transduction
KW - T-Lymphocyte Subsets/immunology
KW - Th1 Cells/immunology
KW - Young Adult
U2 - 10.1111/sji.12835
DO - 10.1111/sji.12835
M3 - Journal article
C2 - 31596502
VL - 91
JO - Scandinavian Journal of Immunology, Supplement
JF - Scandinavian Journal of Immunology, Supplement
SN - 0301-6323
IS - 1
M1 - e12835
ER -
ID: 257035759