Analysis of activated GAPs and GEFs in cell lysates
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Analysis of activated GAPs and GEFs in cell lysates. / García-Mata, Rafael; Wennerberg, Krister; Arthur, William T; Noren, Nicole K; Ellerbroek, Shawn M; Burridge, Keith.
In: Methods in Enzymology, Vol. 406, 2006, p. 425-37.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Analysis of activated GAPs and GEFs in cell lysates
AU - García-Mata, Rafael
AU - Wennerberg, Krister
AU - Arthur, William T
AU - Noren, Nicole K
AU - Ellerbroek, Shawn M
AU - Burridge, Keith
PY - 2006
Y1 - 2006
N2 - An assay was developed that allows the precipitation of the active pools of Rho-GEFs, Rho-GAPs, or effectors from cell or tissue lysates. This assay can be used to identify GEFs, GAPs, and effectors involved in specific cellular pathways to determine their GTPase specificity and to monitor the temporal activation of GEFs and GAPs in response to upstream signals.
AB - An assay was developed that allows the precipitation of the active pools of Rho-GEFs, Rho-GAPs, or effectors from cell or tissue lysates. This assay can be used to identify GEFs, GAPs, and effectors involved in specific cellular pathways to determine their GTPase specificity and to monitor the temporal activation of GEFs and GAPs in response to upstream signals.
KW - Animals
KW - CHO Cells
KW - Cricetinae
KW - GTPase-Activating Proteins/metabolism
KW - Glutathione Transferase/genetics
KW - Guanine Nucleotide Exchange Factors/metabolism
KW - Humans
KW - Mice
KW - NIH 3T3 Cells
KW - Recombinant Fusion Proteins/metabolism
KW - rho GTP-Binding Proteins/genetics
U2 - 10.1016/S0076-6879(06)06031-9
DO - 10.1016/S0076-6879(06)06031-9
M3 - Journal article
C2 - 16472675
VL - 406
SP - 425
EP - 437
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
ER -
ID: 199432674