An isotonic preparation of 1 mg/ml indocyanine green is not toxic to hyperconfluent ARPE19 cells, even after prolonged exposure

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

An isotonic preparation of 1 mg/ml indocyanine green is not toxic to hyperconfluent ARPE19 cells, even after prolonged exposure. / Kiilgaard, Jens Folke; Nissen, Mogens Holst; la Cour, Morten; Kiilgaard, Jens Folke; Nissen, Mogens Holst; la Cour, Morten.

In: Acta Ophthalmologica Scandinavica, Vol. 84, No. 1, 2006, p. 42-6.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kiilgaard, JF, Nissen, MH, la Cour, M, Kiilgaard, JF, Nissen, MH & la Cour, M 2006, 'An isotonic preparation of 1 mg/ml indocyanine green is not toxic to hyperconfluent ARPE19 cells, even after prolonged exposure', Acta Ophthalmologica Scandinavica, vol. 84, no. 1, pp. 42-6. https://doi.org/10.1111/j.1600-0420.2005.00593.x, https://doi.org/10.1111/j.1600-0420.2005.00593.x

APA

Kiilgaard, J. F., Nissen, M. H., la Cour, M., Kiilgaard, J. F., Nissen, M. H., & la Cour, M. (2006). An isotonic preparation of 1 mg/ml indocyanine green is not toxic to hyperconfluent ARPE19 cells, even after prolonged exposure. Acta Ophthalmologica Scandinavica, 84(1), 42-6. https://doi.org/10.1111/j.1600-0420.2005.00593.x, https://doi.org/10.1111/j.1600-0420.2005.00593.x

Vancouver

Kiilgaard JF, Nissen MH, la Cour M, Kiilgaard JF, Nissen MH, la Cour M. An isotonic preparation of 1 mg/ml indocyanine green is not toxic to hyperconfluent ARPE19 cells, even after prolonged exposure. Acta Ophthalmologica Scandinavica. 2006;84(1):42-6. https://doi.org/10.1111/j.1600-0420.2005.00593.x, https://doi.org/10.1111/j.1600-0420.2005.00593.x

Author

Kiilgaard, Jens Folke ; Nissen, Mogens Holst ; la Cour, Morten ; Kiilgaard, Jens Folke ; Nissen, Mogens Holst ; la Cour, Morten. / An isotonic preparation of 1 mg/ml indocyanine green is not toxic to hyperconfluent ARPE19 cells, even after prolonged exposure. In: Acta Ophthalmologica Scandinavica. 2006 ; Vol. 84, No. 1. pp. 42-6.

Bibtex

@article{23246dc0b93811ddae57000ea68e967b,
title = "An isotonic preparation of 1 mg/ml indocyanine green is not toxic to hyperconfluent ARPE19 cells, even after prolonged exposure",
abstract = "PURPOSE: To investigate the in vitro toxicity of indocyanine green and infracyanine green (ICG) to cultured ARPE19 cells, in particular with respect to the concentration and time dependence of this toxicity. METHODS: ARPE19 cells were grown for at least 1 week past confluence (hyperconfluent cells) before being subjected to challenge with ICG. Cell survival was tested with the MTT assay. RESULTS: When applied in isotonic solutions, ICG in all concentrations (below 5 mg/ml) and at all exposure times tested (2 mins-2 hours) was found not to affect the survival of ARPE19 cells. ARPE19 cultures older than 30 days were more resistant to a 5 mg/ml hypotonic ICG solution than younger cultures. CONCLUSION: When toxicity of ICG was tested in hyperconfluent ARPE19 cultures, these cells were found to be more resistant to the dye than has been previously reported for more immature ARPE19 cells.",
author = "Kiilgaard, {Jens Folke} and Nissen, {Mogens Holst} and {la Cour}, Morten and Kiilgaard, {Jens Folke} and Nissen, {Mogens Holst} and {la Cour}, Morten",
note = "Keywords: Cell Line; Cell Survival; Coloring Agents; Humans; Indocyanine Green; Isotonic Solutions; Pigment Epithelium of Eye; Retinal Perforations; Tetrazolium Salts; Thiazoles; Time Factors",
year = "2006",
doi = "10.1111/j.1600-0420.2005.00593.x",
language = "English",
volume = "84",
pages = "42--6",
journal = "Acta Ophthalmologica",
issn = "1755-375X",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - An isotonic preparation of 1 mg/ml indocyanine green is not toxic to hyperconfluent ARPE19 cells, even after prolonged exposure

AU - Kiilgaard, Jens Folke

AU - Nissen, Mogens Holst

AU - la Cour, Morten

AU - Kiilgaard, Jens Folke

AU - Nissen, Mogens Holst

AU - la Cour, Morten

N1 - Keywords: Cell Line; Cell Survival; Coloring Agents; Humans; Indocyanine Green; Isotonic Solutions; Pigment Epithelium of Eye; Retinal Perforations; Tetrazolium Salts; Thiazoles; Time Factors

PY - 2006

Y1 - 2006

N2 - PURPOSE: To investigate the in vitro toxicity of indocyanine green and infracyanine green (ICG) to cultured ARPE19 cells, in particular with respect to the concentration and time dependence of this toxicity. METHODS: ARPE19 cells were grown for at least 1 week past confluence (hyperconfluent cells) before being subjected to challenge with ICG. Cell survival was tested with the MTT assay. RESULTS: When applied in isotonic solutions, ICG in all concentrations (below 5 mg/ml) and at all exposure times tested (2 mins-2 hours) was found not to affect the survival of ARPE19 cells. ARPE19 cultures older than 30 days were more resistant to a 5 mg/ml hypotonic ICG solution than younger cultures. CONCLUSION: When toxicity of ICG was tested in hyperconfluent ARPE19 cultures, these cells were found to be more resistant to the dye than has been previously reported for more immature ARPE19 cells.

AB - PURPOSE: To investigate the in vitro toxicity of indocyanine green and infracyanine green (ICG) to cultured ARPE19 cells, in particular with respect to the concentration and time dependence of this toxicity. METHODS: ARPE19 cells were grown for at least 1 week past confluence (hyperconfluent cells) before being subjected to challenge with ICG. Cell survival was tested with the MTT assay. RESULTS: When applied in isotonic solutions, ICG in all concentrations (below 5 mg/ml) and at all exposure times tested (2 mins-2 hours) was found not to affect the survival of ARPE19 cells. ARPE19 cultures older than 30 days were more resistant to a 5 mg/ml hypotonic ICG solution than younger cultures. CONCLUSION: When toxicity of ICG was tested in hyperconfluent ARPE19 cultures, these cells were found to be more resistant to the dye than has been previously reported for more immature ARPE19 cells.

U2 - 10.1111/j.1600-0420.2005.00593.x

DO - 10.1111/j.1600-0420.2005.00593.x

M3 - Journal article

C2 - 16445438

VL - 84

SP - 42

EP - 46

JO - Acta Ophthalmologica

JF - Acta Ophthalmologica

SN - 1755-375X

IS - 1

ER -

ID: 8724837