An Important Role for RPRD1B in the Heat Shock Response
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
An Important Role for RPRD1B in the Heat Shock Response. / Cugusi, Simona; Bajpe, Prashanth Kumar; Mitter, Richard; Patel, Harshil; Stewart, Aengus; Svejstrup, Jesper Q.
In: Molecular and Cellular Biology, Vol. 42, No. 10, 2022, p. e0017322.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - An Important Role for RPRD1B in the Heat Shock Response
AU - Cugusi, Simona
AU - Bajpe, Prashanth Kumar
AU - Mitter, Richard
AU - Patel, Harshil
AU - Stewart, Aengus
AU - Svejstrup, Jesper Q.
PY - 2022
Y1 - 2022
N2 - During the heat shock response (HSR), heat shock factor (HSF1 in mammals) binds to target gene promoters, resulting in increased expression of heat shock proteins that help maintain protein homeostasis and ensure cell survival. Besides HSF1, only a relatively few transcription factors with a specific role in ensuring correctly regulated gene expression during the HSR have been described. Here, we use proteomic and genomic (CRISPR) screening to identify a role for RPRD1B in the response to heat shock. Indeed, cells depleted for RPRD1B are heat shock sensitive and show decreased expression of key heat shock proteins (HSPs). These results add to our understanding of the connection between basic gene expression mechanisms and the HSR.
AB - During the heat shock response (HSR), heat shock factor (HSF1 in mammals) binds to target gene promoters, resulting in increased expression of heat shock proteins that help maintain protein homeostasis and ensure cell survival. Besides HSF1, only a relatively few transcription factors with a specific role in ensuring correctly regulated gene expression during the HSR have been described. Here, we use proteomic and genomic (CRISPR) screening to identify a role for RPRD1B in the response to heat shock. Indeed, cells depleted for RPRD1B are heat shock sensitive and show decreased expression of key heat shock proteins (HSPs). These results add to our understanding of the connection between basic gene expression mechanisms and the HSR.
KW - heat shock
KW - HSF
KW - HSF1
KW - RNA polymerase II
KW - RPRD1B
KW - transcription
U2 - 10.1128/mcb.00173-22
DO - 10.1128/mcb.00173-22
M3 - Journal article
C2 - 36121223
AN - SCOPUS:85140416639
VL - 42
SP - e0017322
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 10
ER -
ID: 324316062