Amplified fragment length polymorphism and pulsed field gel electrophoresis for subspecies differentiation of Serpulina pilosicoli

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Standard

Amplified fragment length polymorphism and pulsed field gel electrophoresis for subspecies differentiation of Serpulina pilosicoli. / Møller, Kristian; Jensen, Tim K.; Boye, Mette; Leser, Thomas D.; Ahrens, Peter.

In: Anaerobe, Vol. 5, No. 3-4, 06.2000, p. 313-315.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Møller, K, Jensen, TK, Boye, M, Leser, TD & Ahrens, P 2000, 'Amplified fragment length polymorphism and pulsed field gel electrophoresis for subspecies differentiation of Serpulina pilosicoli', Anaerobe, vol. 5, no. 3-4, pp. 313-315. https://doi.org/10.1006/anae.1999.0207

APA

Møller, K., Jensen, T. K., Boye, M., Leser, T. D., & Ahrens, P. (2000). Amplified fragment length polymorphism and pulsed field gel electrophoresis for subspecies differentiation of Serpulina pilosicoli. Anaerobe, 5(3-4), 313-315. https://doi.org/10.1006/anae.1999.0207

Vancouver

Møller K, Jensen TK, Boye M, Leser TD, Ahrens P. Amplified fragment length polymorphism and pulsed field gel electrophoresis for subspecies differentiation of Serpulina pilosicoli. Anaerobe. 2000 Jun;5(3-4):313-315. https://doi.org/10.1006/anae.1999.0207

Author

Møller, Kristian ; Jensen, Tim K. ; Boye, Mette ; Leser, Thomas D. ; Ahrens, Peter. / Amplified fragment length polymorphism and pulsed field gel electrophoresis for subspecies differentiation of Serpulina pilosicoli. In: Anaerobe. 2000 ; Vol. 5, No. 3-4. pp. 313-315.

Bibtex

@article{f1e9af6127964cc6b457c8c5d70f874d,
title = "Amplified fragment length polymorphism and pulsed field gel electrophoresis for subspecies differentiation of Serpulina pilosicoli",
abstract = "Pulsed field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP) were compared for their ability to differentiate between 50 porcine Serpulina pilosicoli isolates. Both techniques were highly sensitive, dividing the isolates into 36 and 38 groups, respectively. Due to the low workload and high capacity and sensitivity of AFLP, this technique is recommended for future studies of S. pilosicoli. S. pilosicoli isolates were genetically diverse, and generally, identical subtypes were only identified in cultures from the same herd. (C) 1999 Academic Press.",
keywords = "AFLP, PFGE, Serpulina pilosicoli, Subtypes",
author = "Kristian M{\o}ller and Jensen, {Tim K.} and Mette Boye and Leser, {Thomas D.} and Peter Ahrens",
year = "2000",
month = jun,
doi = "10.1006/anae.1999.0207",
language = "English",
volume = "5",
pages = "313--315",
journal = "Anaerobe",
issn = "1075-9964",
publisher = "Academic Press",
number = "3-4",

}

RIS

TY - JOUR

T1 - Amplified fragment length polymorphism and pulsed field gel electrophoresis for subspecies differentiation of Serpulina pilosicoli

AU - Møller, Kristian

AU - Jensen, Tim K.

AU - Boye, Mette

AU - Leser, Thomas D.

AU - Ahrens, Peter

PY - 2000/6

Y1 - 2000/6

N2 - Pulsed field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP) were compared for their ability to differentiate between 50 porcine Serpulina pilosicoli isolates. Both techniques were highly sensitive, dividing the isolates into 36 and 38 groups, respectively. Due to the low workload and high capacity and sensitivity of AFLP, this technique is recommended for future studies of S. pilosicoli. S. pilosicoli isolates were genetically diverse, and generally, identical subtypes were only identified in cultures from the same herd. (C) 1999 Academic Press.

AB - Pulsed field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP) were compared for their ability to differentiate between 50 porcine Serpulina pilosicoli isolates. Both techniques were highly sensitive, dividing the isolates into 36 and 38 groups, respectively. Due to the low workload and high capacity and sensitivity of AFLP, this technique is recommended for future studies of S. pilosicoli. S. pilosicoli isolates were genetically diverse, and generally, identical subtypes were only identified in cultures from the same herd. (C) 1999 Academic Press.

KW - AFLP

KW - PFGE

KW - Serpulina pilosicoli

KW - Subtypes

UR - http://www.scopus.com/inward/record.url?scp=0033141197&partnerID=8YFLogxK

U2 - 10.1006/anae.1999.0207

DO - 10.1006/anae.1999.0207

M3 - Journal article

AN - SCOPUS:0033141197

VL - 5

SP - 313

EP - 315

JO - Anaerobe

JF - Anaerobe

SN - 1075-9964

IS - 3-4

ER -

ID: 339895718