Amplified fragment length polymorphism and pulsed field gel electrophoresis for subspecies differentiation of Serpulina pilosicoli
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Amplified fragment length polymorphism and pulsed field gel electrophoresis for subspecies differentiation of Serpulina pilosicoli. / Møller, Kristian; Jensen, Tim K.; Boye, Mette; Leser, Thomas D.; Ahrens, Peter.
In: Anaerobe, Vol. 5, No. 3-4, 06.2000, p. 313-315.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Amplified fragment length polymorphism and pulsed field gel electrophoresis for subspecies differentiation of Serpulina pilosicoli
AU - Møller, Kristian
AU - Jensen, Tim K.
AU - Boye, Mette
AU - Leser, Thomas D.
AU - Ahrens, Peter
PY - 2000/6
Y1 - 2000/6
N2 - Pulsed field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP) were compared for their ability to differentiate between 50 porcine Serpulina pilosicoli isolates. Both techniques were highly sensitive, dividing the isolates into 36 and 38 groups, respectively. Due to the low workload and high capacity and sensitivity of AFLP, this technique is recommended for future studies of S. pilosicoli. S. pilosicoli isolates were genetically diverse, and generally, identical subtypes were only identified in cultures from the same herd. (C) 1999 Academic Press.
AB - Pulsed field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP) were compared for their ability to differentiate between 50 porcine Serpulina pilosicoli isolates. Both techniques were highly sensitive, dividing the isolates into 36 and 38 groups, respectively. Due to the low workload and high capacity and sensitivity of AFLP, this technique is recommended for future studies of S. pilosicoli. S. pilosicoli isolates were genetically diverse, and generally, identical subtypes were only identified in cultures from the same herd. (C) 1999 Academic Press.
KW - AFLP
KW - PFGE
KW - Serpulina pilosicoli
KW - Subtypes
UR - http://www.scopus.com/inward/record.url?scp=0033141197&partnerID=8YFLogxK
U2 - 10.1006/anae.1999.0207
DO - 10.1006/anae.1999.0207
M3 - Journal article
AN - SCOPUS:0033141197
VL - 5
SP - 313
EP - 315
JO - Anaerobe
JF - Anaerobe
SN - 1075-9964
IS - 3-4
ER -
ID: 339895718