Alveolar cytokines and interferon autoantibodies in COVID-19 ARDS
Research output: Contribution to journal › Journal article › Research › peer-review
Documents
- Fulltext
Final published version, 942 KB, PDF document
Background: Type I interferon (IFN-I) and IFN autoantibodies play a crucial role in controlling SARS-CoV-2 infection. The levels of these mediators have only rarely been studied in the alveolar compartment in patients with COVID-19 acute respiratory distress syndrome (CARDS) but have not been compared across different ARDS etiologies, and the potential effect of dexamethasone (DXM) on these mediators is not known. Methods: We assessed the integrity of the alveolo-capillary membrane, interleukins, type I, II, and III IFNs, and IFN autoantibodies by studying the epithelial lining fluid (ELF) volumes, alveolar concentration of protein, and ELF-corrected concentrations of cytokines in two patient subgroups and controls. Results: A total of 16 patients with CARDS (four without and 12 with DXM treatment), eight with non-CARDS, and 15 healthy controls were included. The highest ELF volumes and protein levels were observed in CARDS. Systemic and ELF-corrected alveolar concentrations of interleukin (IL)-6 appeared to be particularly low in patients with CARDS receiving DXM, whereas alveolar levels of IL-8 were high regardless of DXM treatment. Alveolar levels of IFNs were similar between CARDS and non-CARDS patients, and IFNα and IFNω autoantibody levels were higher in patients with CARDS and non-CARDS than in healthy controls. Conclusions: Patients with CARDS exhibited greater alveolo-capillary barrier disruption with compartmentalization of IL-8, regardless of DXM treatment, whereas systemic and alveolar levels of IL-6 were lower in the DXM-treated subgroup. IFN-I autoantibodies were higher in the BALF of CARDS patients, independent of DXM, whereas IFN autoantibodies in plasma were similar to those in controls.
Original language | English |
---|---|
Article number | 1353012 |
Journal | Frontiers in Immunology |
Volume | 15 |
Number of pages | 9 |
ISSN | 1664-3224 |
DOIs | |
Publication status | Published - 2024 |
Bibliographical note
Publisher Copyright:
Copyright © 2024 Jonassen, Jørgensen, Mitchell, Mogensen, Berg, Ronit and Plovsing.
- acute respiratory distress syndrome, autoantibodies, bronchoalveolar lavage fluid, coronavirus disease 2019, inflammation, interferons
Research areas
ID: 388016102