Allele-Specific DNA Methylation Detection by Pyrosequencing®

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Allele-Specific DNA Methylation Detection by Pyrosequencing®. / Sommer Kristensen, Lasse; Johansen, Jens Vilstrup; Grønbæk, Kirsten.

Pyrosequencing: Methods and Protocols. ed. / Ulrich Lehmann; Jörg Tost. Vol. 1315 2015. p. 271-89 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Sommer Kristensen, L, Johansen, JV & Grønbæk, K 2015, Allele-Specific DNA Methylation Detection by Pyrosequencing®. in U Lehmann & J Tost (eds), Pyrosequencing: Methods and Protocols. vol. 1315, Methods in molecular biology (Clifton, N.J.), pp. 271-89. https://doi.org/10.1007/978-1-4939-2715-9_20

APA

Sommer Kristensen, L., Johansen, J. V., & Grønbæk, K. (2015). Allele-Specific DNA Methylation Detection by Pyrosequencing®. In U. Lehmann, & J. Tost (Eds.), Pyrosequencing: Methods and Protocols (Vol. 1315, pp. 271-89). Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-4939-2715-9_20

Vancouver

Sommer Kristensen L, Johansen JV, Grønbæk K. Allele-Specific DNA Methylation Detection by Pyrosequencing®. In Lehmann U, Tost J, editors, Pyrosequencing: Methods and Protocols. Vol. 1315. 2015. p. 271-89. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-4939-2715-9_20

Author

Sommer Kristensen, Lasse ; Johansen, Jens Vilstrup ; Grønbæk, Kirsten. / Allele-Specific DNA Methylation Detection by Pyrosequencing®. Pyrosequencing: Methods and Protocols. editor / Ulrich Lehmann ; Jörg Tost. Vol. 1315 2015. pp. 271-89 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{8b0a529948a340a99c0dc2218016d0c6,
title = "Allele-Specific DNA Methylation Detection by Pyrosequencing{\textregistered}",
abstract = "DNA methylation is an epigenetic modification that plays important roles in healthy as well as diseased cells, by influencing the transcription of genes. In spite the fact that human somatic cells are diploid, most of the currently available methods for the study of DNA methylation do not provide information on the methylation status of individual alleles of genes. This information may be of importance in many situations. In particular, in cancer both alleles of tumour suppressor genes generally need to be inactivated for a phenotypic effect to be observed. Here, we present a simple and cost-effective protocol for allele-specific DNA methylation detection based on Pyrosequencing({\textregistered}) of methylation-specific PCR (MSP) products including a single nucleotide polymorphism (SNP) within the amplicon.",
keywords = "Alleles, DNA, DNA Methylation, Humans, Polymerase Chain Reaction, Sequence Analysis, DNA, Sulfites",
author = "{Sommer Kristensen}, Lasse and Johansen, {Jens Vilstrup} and Kirsten Gr{\o}nb{\ae}k",
year = "2015",
doi = "10.1007/978-1-4939-2715-9_20",
language = "English",
isbn = "978-1-4939-2714-2",
volume = "1315",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "271--89",
editor = "Ulrich Lehmann and J{\"o}rg Tost",
booktitle = "Pyrosequencing",

}

RIS

TY - CHAP

T1 - Allele-Specific DNA Methylation Detection by Pyrosequencing®

AU - Sommer Kristensen, Lasse

AU - Johansen, Jens Vilstrup

AU - Grønbæk, Kirsten

PY - 2015

Y1 - 2015

N2 - DNA methylation is an epigenetic modification that plays important roles in healthy as well as diseased cells, by influencing the transcription of genes. In spite the fact that human somatic cells are diploid, most of the currently available methods for the study of DNA methylation do not provide information on the methylation status of individual alleles of genes. This information may be of importance in many situations. In particular, in cancer both alleles of tumour suppressor genes generally need to be inactivated for a phenotypic effect to be observed. Here, we present a simple and cost-effective protocol for allele-specific DNA methylation detection based on Pyrosequencing(®) of methylation-specific PCR (MSP) products including a single nucleotide polymorphism (SNP) within the amplicon.

AB - DNA methylation is an epigenetic modification that plays important roles in healthy as well as diseased cells, by influencing the transcription of genes. In spite the fact that human somatic cells are diploid, most of the currently available methods for the study of DNA methylation do not provide information on the methylation status of individual alleles of genes. This information may be of importance in many situations. In particular, in cancer both alleles of tumour suppressor genes generally need to be inactivated for a phenotypic effect to be observed. Here, we present a simple and cost-effective protocol for allele-specific DNA methylation detection based on Pyrosequencing(®) of methylation-specific PCR (MSP) products including a single nucleotide polymorphism (SNP) within the amplicon.

KW - Alleles

KW - DNA

KW - DNA Methylation

KW - Humans

KW - Polymerase Chain Reaction

KW - Sequence Analysis, DNA

KW - Sulfites

U2 - 10.1007/978-1-4939-2715-9_20

DO - 10.1007/978-1-4939-2715-9_20

M3 - Book chapter

C2 - 26103906

SN - 978-1-4939-2714-2

VL - 1315

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 271

EP - 289

BT - Pyrosequencing

A2 - Lehmann, Ulrich

A2 - Tost, Jörg

ER -

ID: 168638799