Administration of whey protein complexed vitamin D3 to vitamin D3-deficient growing Sprague-Dawley rats
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Administration of whey protein complexed vitamin D3 to vitamin D3-deficient growing Sprague-Dawley rats. / Jakobsen, Louise M. Arildsen; He, Weiwei; Ditzel, Nicholas; Danielsen, Marianne; Dalsgaard, Trine K.; Jørgensen, Niklas Rye; Bollen, Peter; Bertram, Hanne C.
In: Food and Function, Vol. 13, No. 8, 2022, p. 4691-4698.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Administration of whey protein complexed vitamin D3 to vitamin D3-deficient growing Sprague-Dawley rats
AU - Jakobsen, Louise M. Arildsen
AU - He, Weiwei
AU - Ditzel, Nicholas
AU - Danielsen, Marianne
AU - Dalsgaard, Trine K.
AU - Jørgensen, Niklas Rye
AU - Bollen, Peter
AU - Bertram, Hanne C.
N1 - Publisher Copyright: © 2022 The Royal Society of Chemistry.
PY - 2022
Y1 - 2022
N2 - Vitamin D deficiency is a global health issue with consequences for bone health. Complexation of vitamin D3 with specific whey proteins might increase the bioavailability and enhance the effect of dietary supplementation on health outcomes. The current rat study was set up to investigate if complexation of vitamin D3 with whey protein isolate (WPI) or β-lactoglobulin (B-LG) increases bioavailability of the vitamin and how it impacts markers of bone turnover and bone structure. For 8 weeks, growing male Sprague Dawley rats (n = 48) were fed a vitamin D-deficient diet and during the final 4 weeks gavage dosing of vitamin D3 either alone (VitD) or complexed with WPI (VitD + WPI) or β-LG (VitD + B-LG) was administered. A placebo treatment (placebo) was also included. After sacrifice, samples of bone were collected and analyzed using biomechanical testing and μCT scanning. The concentrations of vitamin D3, vitamin D3 metabolites and bone markers (P1NP and CTX) were measured in serum. The results showed that VitD + B-LG appeared to induce lower levels of 25-hydroxy vitamin D3 in serum compared to VitD alone. Markers of bone turnover were generally higher in the VitD group compared to placebo and the VitD + WPI and VitD + B-LG treatments. No effects of treatments on bone strength or bone microstructure were detected. In conclusion, whey protein complexation of vitamin D3 supplements appeared to have no beneficial effects on circulating vitamin D3 metabolites but this did not impose changes in bone strength or trabecular bone microstructure.
AB - Vitamin D deficiency is a global health issue with consequences for bone health. Complexation of vitamin D3 with specific whey proteins might increase the bioavailability and enhance the effect of dietary supplementation on health outcomes. The current rat study was set up to investigate if complexation of vitamin D3 with whey protein isolate (WPI) or β-lactoglobulin (B-LG) increases bioavailability of the vitamin and how it impacts markers of bone turnover and bone structure. For 8 weeks, growing male Sprague Dawley rats (n = 48) were fed a vitamin D-deficient diet and during the final 4 weeks gavage dosing of vitamin D3 either alone (VitD) or complexed with WPI (VitD + WPI) or β-LG (VitD + B-LG) was administered. A placebo treatment (placebo) was also included. After sacrifice, samples of bone were collected and analyzed using biomechanical testing and μCT scanning. The concentrations of vitamin D3, vitamin D3 metabolites and bone markers (P1NP and CTX) were measured in serum. The results showed that VitD + B-LG appeared to induce lower levels of 25-hydroxy vitamin D3 in serum compared to VitD alone. Markers of bone turnover were generally higher in the VitD group compared to placebo and the VitD + WPI and VitD + B-LG treatments. No effects of treatments on bone strength or bone microstructure were detected. In conclusion, whey protein complexation of vitamin D3 supplements appeared to have no beneficial effects on circulating vitamin D3 metabolites but this did not impose changes in bone strength or trabecular bone microstructure.
KW - Animals
KW - Biomarkers
KW - Cholecalciferol
KW - Dietary Supplements
KW - Male
KW - Rats
KW - Rats, Sprague-Dawley
KW - Vitamin D
KW - Vitamin D Deficiency/drug therapy
KW - Vitamins/therapeutic use
KW - Whey Proteins/therapeutic use
U2 - 10.1039/d1fo03645a
DO - 10.1039/d1fo03645a
M3 - Journal article
C2 - 35379998
AN - SCOPUS:85128800163
VL - 13
SP - 4691
EP - 4698
JO - Food & Function
JF - Food & Function
SN - 2042-6496
IS - 8
ER -
ID: 307753606