Acute exercise remodels promoter methylation in human skeletal muscle
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Acute exercise remodels promoter methylation in human skeletal muscle. / Barrès, Romain; Yan, Jie; Egan, Brendan; Treebak, Jonas Thue; Rasmussen, Morten; Fritz, Tomas; Caidahl, Kenneth; Krook, Anna; O'Gorman, Donal J; Zierath, Juleen R.
In: Cell Metabolism, Vol. 15, No. 3, 07.03.2012, p. 405-11.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Acute exercise remodels promoter methylation in human skeletal muscle
AU - Barrès, Romain
AU - Yan, Jie
AU - Egan, Brendan
AU - Treebak, Jonas Thue
AU - Rasmussen, Morten
AU - Fritz, Tomas
AU - Caidahl, Kenneth
AU - Krook, Anna
AU - O'Gorman, Donal J
AU - Zierath, Juleen R
N1 - Copyright © 2012 Elsevier Inc. All rights reserved.
PY - 2012/3/7
Y1 - 2012/3/7
N2 - DNA methylation is a covalent biochemical modification controlling chromatin structure and gene expression. Exercise elicits gene expression changes that trigger structural and metabolic adaptations in skeletal muscle. We determined whether DNA methylation plays a role in exercise-induced gene expression. Whole genome methylation was decreased in skeletal muscle biopsies obtained from healthy sedentary men and women after acute exercise. Exercise induced a dose-dependent expression of PGC-1a, PDK4, and PPAR-d, together with a marked hypomethylation on each respective promoter. Similarly, promoter methylation of PGC-1a, PDK4, and PPAR-d was markedly decreased in mouse soleus muscles 45 min after ex vivo contraction. In L6 myotubes, caffeine exposure induced gene hypomethylation in parallel with an increase in the respective mRNA content. Collectively, our results provide evidence that acute gene activation is associated with a dynamic change in DNA methylation in skeletal muscle and suggest that DNA hypomethylation is an early event in contraction-induced gene activation.
AB - DNA methylation is a covalent biochemical modification controlling chromatin structure and gene expression. Exercise elicits gene expression changes that trigger structural and metabolic adaptations in skeletal muscle. We determined whether DNA methylation plays a role in exercise-induced gene expression. Whole genome methylation was decreased in skeletal muscle biopsies obtained from healthy sedentary men and women after acute exercise. Exercise induced a dose-dependent expression of PGC-1a, PDK4, and PPAR-d, together with a marked hypomethylation on each respective promoter. Similarly, promoter methylation of PGC-1a, PDK4, and PPAR-d was markedly decreased in mouse soleus muscles 45 min after ex vivo contraction. In L6 myotubes, caffeine exposure induced gene hypomethylation in parallel with an increase in the respective mRNA content. Collectively, our results provide evidence that acute gene activation is associated with a dynamic change in DNA methylation in skeletal muscle and suggest that DNA hypomethylation is an early event in contraction-induced gene activation.
KW - Adult
KW - Animals
KW - DNA Methylation
KW - Exercise
KW - Female
KW - Humans
KW - Male
KW - Mice
KW - Mice, Inbred C57BL
KW - Muscle, Skeletal
KW - Young Adult
U2 - 10.1016/j.cmet.2012.01.001
DO - 10.1016/j.cmet.2012.01.001
M3 - Journal article
C2 - 22405075
VL - 15
SP - 405
EP - 411
JO - Cell Metabolism
JF - Cell Metabolism
SN - 1550-4131
IS - 3
ER -
ID: 45132509