Actinobacillus pleuropneumoniae Osteomyelitis in Pigs Demonstrated by Fluorescent in Situ Hybridization
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Actinobacillus pleuropneumoniae Osteomyelitis in Pigs Demonstrated by Fluorescent in Situ Hybridization. / Jensen, T. K.; Boye, M.; Hagedorn-Olsen, T.; Riising, H. J.; Angen.
In: Veterinary Pathology, Vol. 36, No. 3, 05.1999, p. 258-261.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Actinobacillus pleuropneumoniae Osteomyelitis in Pigs Demonstrated by Fluorescent in Situ Hybridization
AU - Jensen, T. K.
AU - Boye, M.
AU - Hagedorn-Olsen, T.
AU - Riising, H. J.
AU - Angen, null
N1 - Funding Information: We thank A. R. Pedersen, U. L. Andreasen, and T. Plambeck for technical assistance. This work was supported by the Research Secretariat of the Ministry of Food, Agriculture and Fisheries.
PY - 1999/5
Y1 - 1999/5
N2 - Necrotizing osteomyelitis and fibrinopurulent arthritis with isolation of Actinobacillus pleuropneumoniae serotype 2 is reported in two pigs from a herd with lameness and mild coughing problems among 8 to 12-week-old pigs. Application of fluorescent in situ hybridization targeting 16S ribosomal RNA of A. pleuropneumoniae in formalin-fixed tissue was performed to verify the association of A. pleuropneumoniae with the bone and joint lesions. By in situ hybridization A. pleuropneumoniae was demonstrated as multiple microcolonies or single cells dispersed in focal fibrinonecrotizing pleuropneumonia, in joints with arthritis, and in bone necroses including lysis of growth plate and suppurative inflammation in the adjacent trabecular metaphysis, thus demonstrating that well-known infections manifest new, unusual lesions.
AB - Necrotizing osteomyelitis and fibrinopurulent arthritis with isolation of Actinobacillus pleuropneumoniae serotype 2 is reported in two pigs from a herd with lameness and mild coughing problems among 8 to 12-week-old pigs. Application of fluorescent in situ hybridization targeting 16S ribosomal RNA of A. pleuropneumoniae in formalin-fixed tissue was performed to verify the association of A. pleuropneumoniae with the bone and joint lesions. By in situ hybridization A. pleuropneumoniae was demonstrated as multiple microcolonies or single cells dispersed in focal fibrinonecrotizing pleuropneumonia, in joints with arthritis, and in bone necroses including lysis of growth plate and suppurative inflammation in the adjacent trabecular metaphysis, thus demonstrating that well-known infections manifest new, unusual lesions.
KW - Actinobacillus pleuropneumoniae
KW - Arthritis
KW - In situ hybridization
KW - Osteomyelitis
KW - Pigs
UR - http://www.scopus.com/inward/record.url?scp=0033129668&partnerID=8YFLogxK
U2 - 10.1354/vp.36-3-258
DO - 10.1354/vp.36-3-258
M3 - Journal article
C2 - 10332836
AN - SCOPUS:0033129668
VL - 36
SP - 258
EP - 261
JO - Veterinary Pathology
JF - Veterinary Pathology
SN - 0300-9858
IS - 3
ER -
ID: 339895833