Actin maturation requires the ACTMAP/C19orf54 protease

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Actin maturation requires the ACTMAP/C19orf54 protease. / Haahr, Peter; Galli, Ricardo A.; van den Hengel, Lisa G.; Bleijerveld, Onno B.; Kazokaitė-Adomaitienė, Justina; Song, Ji Ying; Kroese, Lona J.; Krimpenfort, Paul; Baltissen, Marijke P.; Vermeulen, Michiel; Ottenheijm, Coen A.C.; Brummelkamp, Thijn R.

In: Science, Vol. 377, No. 6614, 2022, p. 1533-1537.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Haahr, P, Galli, RA, van den Hengel, LG, Bleijerveld, OB, Kazokaitė-Adomaitienė, J, Song, JY, Kroese, LJ, Krimpenfort, P, Baltissen, MP, Vermeulen, M, Ottenheijm, CAC & Brummelkamp, TR 2022, 'Actin maturation requires the ACTMAP/C19orf54 protease', Science, vol. 377, no. 6614, pp. 1533-1537. https://doi.org/10.1126/science.abq5082

APA

Haahr, P., Galli, R. A., van den Hengel, L. G., Bleijerveld, O. B., Kazokaitė-Adomaitienė, J., Song, J. Y., Kroese, L. J., Krimpenfort, P., Baltissen, M. P., Vermeulen, M., Ottenheijm, C. A. C., & Brummelkamp, T. R. (2022). Actin maturation requires the ACTMAP/C19orf54 protease. Science, 377(6614), 1533-1537. https://doi.org/10.1126/science.abq5082

Vancouver

Haahr P, Galli RA, van den Hengel LG, Bleijerveld OB, Kazokaitė-Adomaitienė J, Song JY et al. Actin maturation requires the ACTMAP/C19orf54 protease. Science. 2022;377(6614):1533-1537. https://doi.org/10.1126/science.abq5082

Author

Haahr, Peter ; Galli, Ricardo A. ; van den Hengel, Lisa G. ; Bleijerveld, Onno B. ; Kazokaitė-Adomaitienė, Justina ; Song, Ji Ying ; Kroese, Lona J. ; Krimpenfort, Paul ; Baltissen, Marijke P. ; Vermeulen, Michiel ; Ottenheijm, Coen A.C. ; Brummelkamp, Thijn R. / Actin maturation requires the ACTMAP/C19orf54 protease. In: Science. 2022 ; Vol. 377, No. 6614. pp. 1533-1537.

Bibtex

@article{75a708da14cb4513ab104deb20faa98b,
title = "Actin maturation requires the ACTMAP/C19orf54 protease",
abstract = "Protein synthesis generally starts with a methionine that is removed during translation. However, cytoplasmic actin defies this rule because its synthesis involves noncanonical excision of the acetylated methionine by an unidentified enzyme after translation. Here, we identified C19orf54, named ACTMAP (actin maturation protease), as this enzyme. Its ablation resulted in viable mice in which the cytoskeleton was composed of immature actin molecules across all tissues. However, in skeletal muscle, the lengths of sarcomeric actin filaments were shorter, muscle function was decreased, and centralized nuclei, a common hallmark of myopathies, progressively accumulated. Thus, ACTMAP encodes the missing factor required for the synthesis of mature actin and regulates specific actin-dependent traits in vivo.",
author = "Peter Haahr and Galli, {Ricardo A.} and {van den Hengel}, {Lisa G.} and Bleijerveld, {Onno B.} and Justina Kazokaitė-Adomaitienė and Song, {Ji Ying} and Kroese, {Lona J.} and Paul Krimpenfort and Baltissen, {Marijke P.} and Michiel Vermeulen and Ottenheijm, {Coen A.C.} and Brummelkamp, {Thijn R.}",
year = "2022",
doi = "10.1126/science.abq5082",
language = "English",
volume = "377",
pages = "1533--1537",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6614",

}

RIS

TY - JOUR

T1 - Actin maturation requires the ACTMAP/C19orf54 protease

AU - Haahr, Peter

AU - Galli, Ricardo A.

AU - van den Hengel, Lisa G.

AU - Bleijerveld, Onno B.

AU - Kazokaitė-Adomaitienė, Justina

AU - Song, Ji Ying

AU - Kroese, Lona J.

AU - Krimpenfort, Paul

AU - Baltissen, Marijke P.

AU - Vermeulen, Michiel

AU - Ottenheijm, Coen A.C.

AU - Brummelkamp, Thijn R.

PY - 2022

Y1 - 2022

N2 - Protein synthesis generally starts with a methionine that is removed during translation. However, cytoplasmic actin defies this rule because its synthesis involves noncanonical excision of the acetylated methionine by an unidentified enzyme after translation. Here, we identified C19orf54, named ACTMAP (actin maturation protease), as this enzyme. Its ablation resulted in viable mice in which the cytoskeleton was composed of immature actin molecules across all tissues. However, in skeletal muscle, the lengths of sarcomeric actin filaments were shorter, muscle function was decreased, and centralized nuclei, a common hallmark of myopathies, progressively accumulated. Thus, ACTMAP encodes the missing factor required for the synthesis of mature actin and regulates specific actin-dependent traits in vivo.

AB - Protein synthesis generally starts with a methionine that is removed during translation. However, cytoplasmic actin defies this rule because its synthesis involves noncanonical excision of the acetylated methionine by an unidentified enzyme after translation. Here, we identified C19orf54, named ACTMAP (actin maturation protease), as this enzyme. Its ablation resulted in viable mice in which the cytoskeleton was composed of immature actin molecules across all tissues. However, in skeletal muscle, the lengths of sarcomeric actin filaments were shorter, muscle function was decreased, and centralized nuclei, a common hallmark of myopathies, progressively accumulated. Thus, ACTMAP encodes the missing factor required for the synthesis of mature actin and regulates specific actin-dependent traits in vivo.

U2 - 10.1126/science.abq5082

DO - 10.1126/science.abq5082

M3 - Journal article

C2 - 36173861

AN - SCOPUS:85138973746

VL - 377

SP - 1533

EP - 1537

JO - Science

JF - Science

SN - 0036-8075

IS - 6614

ER -

ID: 322282454