Accessible high-speed image-activated cell sorting

Research output: Contribution to journal › Review › Research › peer-review

Standard

Accessible high-speed image-activated cell sorting. / Kuhn, Terra M.; Paulsen, Malte; Cuylen-Haering, Sara.

In: Trends in Cell Biology, 2024.

Research output: Contribution to journal › Review › Research › peer-review

Harvard

Kuhn, TM, Paulsen, M & Cuylen-Haering, S 2024, 'Accessible high-speed image-activated cell sorting', Trends in Cell Biology. https://doi.org/10.1016/j.tcb.2024.04.007

APA

Kuhn, T. M., Paulsen, M., & Cuylen-Haering, S. (Accepted/In press). Accessible high-speed image-activated cell sorting. Trends in Cell Biology. https://doi.org/10.1016/j.tcb.2024.04.007

Vancouver

Kuhn TM, Paulsen M, Cuylen-Haering S. Accessible high-speed image-activated cell sorting. Trends in Cell Biology. 2024. https://doi.org/10.1016/j.tcb.2024.04.007

Author

Kuhn, Terra M. ; Paulsen, Malte ; Cuylen-Haering, Sara. / Accessible high-speed image-activated cell sorting. In: Trends in Cell Biology. 2024.

Bibtex

@article{568455df528142cda80d2530c5ca2bcc,
title = "Accessible high-speed image-activated cell sorting",
abstract = "Over the past six decades, fluorescence-activated cell sorting (FACS) has become an essential technology for basic and clinical research by enabling the isolation of cells of interest in high throughput. Recent technological advancements have started a new era of flow cytometry. By combining the spatial resolution of microscopy with high-speed cell sorting, new instruments allow cell sorting based on simple image-derived parameters or sophisticated image analysis algorithms, thereby greatly expanding the scope of applications. In this review, we discuss the systems that are commercially available or have been described in enough methodological and engineering detail to allow their replication. We summarize their strengths and limitations and highlight applications that have the potential to transform various fields in basic life science research and clinical settings.",
keywords = "cell sorting, flow cytometry, high-throughput imaging, image analysis",
author = "Kuhn, {Terra M.} and Malte Paulsen and Sara Cuylen-Haering",
note = "Publisher Copyright: {\textcopyright} 2024 The Authors",
year = "2024",
doi = "10.1016/j.tcb.2024.04.007",
language = "English",
journal = "Trends in Cell Biology",
issn = "0962-8924",
publisher = "Elsevier Ltd. * Trends Journals",

}

RIS

TY - JOUR

T1 - Accessible high-speed image-activated cell sorting

AU - Kuhn, Terra M.

AU - Paulsen, Malte

AU - Cuylen-Haering, Sara

N1 - Publisher Copyright: © 2024 The Authors

PY - 2024

Y1 - 2024

N2 - Over the past six decades, fluorescence-activated cell sorting (FACS) has become an essential technology for basic and clinical research by enabling the isolation of cells of interest in high throughput. Recent technological advancements have started a new era of flow cytometry. By combining the spatial resolution of microscopy with high-speed cell sorting, new instruments allow cell sorting based on simple image-derived parameters or sophisticated image analysis algorithms, thereby greatly expanding the scope of applications. In this review, we discuss the systems that are commercially available or have been described in enough methodological and engineering detail to allow their replication. We summarize their strengths and limitations and highlight applications that have the potential to transform various fields in basic life science research and clinical settings.

AB - Over the past six decades, fluorescence-activated cell sorting (FACS) has become an essential technology for basic and clinical research by enabling the isolation of cells of interest in high throughput. Recent technological advancements have started a new era of flow cytometry. By combining the spatial resolution of microscopy with high-speed cell sorting, new instruments allow cell sorting based on simple image-derived parameters or sophisticated image analysis algorithms, thereby greatly expanding the scope of applications. In this review, we discuss the systems that are commercially available or have been described in enough methodological and engineering detail to allow their replication. We summarize their strengths and limitations and highlight applications that have the potential to transform various fields in basic life science research and clinical settings.

KW - cell sorting

KW - flow cytometry

KW - high-throughput imaging

KW - image analysis

U2 - 10.1016/j.tcb.2024.04.007

DO - 10.1016/j.tcb.2024.04.007

M3 - Review

C2 - 38789300

AN - SCOPUS:85194032146

JO - Trends in Cell Biology

JF - Trends in Cell Biology

SN - 0962-8924

ER -

ID: 394477016