Absorption and excretion of black currant anthocyanins in humans and watanabe heritable hyperlipidemic rabbits
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Absorption and excretion of black currant anthocyanins in humans and watanabe heritable hyperlipidemic rabbits. / Nielsen, Inge L Finné; Dragsted, Lars Ove; Ravn-Haren, Gitte; Freese, Riitta; Rasmussen, Salka Elbøl.
In: Journal of Agricultural and Food Chemistry, Vol. 51, No. 9, 2003, p. 2813-2820.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Absorption and excretion of black currant anthocyanins in humans and watanabe heritable hyperlipidemic rabbits
AU - Nielsen, Inge L Finné
AU - Dragsted, Lars Ove
AU - Ravn-Haren, Gitte
AU - Freese, Riitta
AU - Rasmussen, Salka Elbøl
N1 - (Ekstern)
PY - 2003
Y1 - 2003
N2 - Anthocyanins are thought to protect against cardiovascular diseases. Watanabe heritable hyperlipidemic (WHHL) rabbits are hypercholesterolemic and used as a model of the development of atherosclerosis. To compare the uptake and excretion of anthocyanins in humans and WHHL rabbits, single-dose black currant anthocyanin studies were performed. Procedures for workup and analyses of urine and plasma samples containing anthocyanins were developed with high recoveries (99 and 81%, respectively) and low limits of quantification (≥6.6 and ≥1.1 nM, respectively). The excretion and absorption of anthocyanins from black currant juice were found to be within the same order of magnitude in the two species regarding urinary excretion within the first 4 h (rabbits, 0.035%; humans, 0.072%) and tmax (rabbits, ∼30 min; humans, ∼45 min). A food matrix effect was detected in rabbits, resulting in the absorption of a higher proportion of the anthocyanins from black currant juice than from an aqueous citric acid matrix. In humans the absorption and urinary excretion of anthocyanins from black currant juice were found to be proportional with dose and not influenced by the ingestion of a rice cake. In both species a larger proportion of the anthocyanin rutinosides than of the glucosides was absorbed, whereas the structure of the aglycon had no influence on the absorption and excretion. The anthocyanins had no effect in rabbits on the antioxidant capacity of plasma measured as Trolox equivalent antioxidant capacity and ferruc reducing ability of plasma.
AB - Anthocyanins are thought to protect against cardiovascular diseases. Watanabe heritable hyperlipidemic (WHHL) rabbits are hypercholesterolemic and used as a model of the development of atherosclerosis. To compare the uptake and excretion of anthocyanins in humans and WHHL rabbits, single-dose black currant anthocyanin studies were performed. Procedures for workup and analyses of urine and plasma samples containing anthocyanins were developed with high recoveries (99 and 81%, respectively) and low limits of quantification (≥6.6 and ≥1.1 nM, respectively). The excretion and absorption of anthocyanins from black currant juice were found to be within the same order of magnitude in the two species regarding urinary excretion within the first 4 h (rabbits, 0.035%; humans, 0.072%) and tmax (rabbits, ∼30 min; humans, ∼45 min). A food matrix effect was detected in rabbits, resulting in the absorption of a higher proportion of the anthocyanins from black currant juice than from an aqueous citric acid matrix. In humans the absorption and urinary excretion of anthocyanins from black currant juice were found to be proportional with dose and not influenced by the ingestion of a rice cake. In both species a larger proportion of the anthocyanin rutinosides than of the glucosides was absorbed, whereas the structure of the aglycon had no influence on the absorption and excretion. The anthocyanins had no effect in rabbits on the antioxidant capacity of plasma measured as Trolox equivalent antioxidant capacity and ferruc reducing ability of plasma.
KW - Anthocyanins
KW - Black currant
KW - HPLC
KW - Humans
KW - Plasma
KW - Ribes nigrum L.
KW - Urine
KW - WHHL rabbits
UR - http://www.scopus.com/inward/record.url?scp=0037462059&partnerID=8YFLogxK
U2 - 10.1021/jf025947u
DO - 10.1021/jf025947u
M3 - Journal article
C2 - 12696978
AN - SCOPUS:0037462059
VL - 51
SP - 2813
EP - 2820
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 9
ER -
ID: 254728421