A Rad26-Def1 complex coordinates repair and RNA pol II proteolysis in response to DNA damage
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A Rad26-Def1 complex coordinates repair and RNA pol II proteolysis in response to DNA damage. / Woudstra, Elies C.; Gilbert, Chris; Fellows, Jane; Jansen, Lars; Brouwer, Jaap; Erdjument-Bromage, Hediye; Tempst, Paul; Svejstrup, Jesper Q.
In: Nature, Vol. 415, No. 6874, 2002, p. 929-933.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A Rad26-Def1 complex coordinates repair and RNA pol II proteolysis in response to DNA damage
AU - Woudstra, Elies C.
AU - Gilbert, Chris
AU - Fellows, Jane
AU - Jansen, Lars
AU - Brouwer, Jaap
AU - Erdjument-Bromage, Hediye
AU - Tempst, Paul
AU - Svejstrup, Jesper Q.
PY - 2002
Y1 - 2002
N2 - Eukaryotic cells use multiple, highly conserved mechanisms to contend with ultraviolet-light-induced DNA damage. One important response mechanism is transcription-coupled repair (TCR), during which DNA lesions in the transcribed strand of an active gene are repaired much faster than in the genome overall. In mammalian cells, defective TCR gives rise to the severe human disorder Cockayne's syndrome (CS). The best-studied CS gene, CSB, codes for a Swi/Snf-like DNA-dependent ATPase, whose yeast homologue is called Rad26 (ref. 4). Here we identify a yeast protein, termed Def1, which forms a complex with Rad26 in chromatin. The phenotypes of cells lacking DEF1 are consistent with a role for this factor in the DNA damage response, but Def1 is not required for TCR. Rather, def1 cells are compromised for transcript elongation, and are unable to degrade RNA polymerase II (RNAPII) in response to DNA damage. Our data suggest that RNAPII stalled at a DNA lesion triggers a coordinated rescue mechanism that requires the Rad26-Def1 complex, and that Def1 enables ubiquitination and proteolysis of RNAPII when the lesion cannot be rapidly removed by Rad26-promoted DNA repair.
AB - Eukaryotic cells use multiple, highly conserved mechanisms to contend with ultraviolet-light-induced DNA damage. One important response mechanism is transcription-coupled repair (TCR), during which DNA lesions in the transcribed strand of an active gene are repaired much faster than in the genome overall. In mammalian cells, defective TCR gives rise to the severe human disorder Cockayne's syndrome (CS). The best-studied CS gene, CSB, codes for a Swi/Snf-like DNA-dependent ATPase, whose yeast homologue is called Rad26 (ref. 4). Here we identify a yeast protein, termed Def1, which forms a complex with Rad26 in chromatin. The phenotypes of cells lacking DEF1 are consistent with a role for this factor in the DNA damage response, but Def1 is not required for TCR. Rather, def1 cells are compromised for transcript elongation, and are unable to degrade RNA polymerase II (RNAPII) in response to DNA damage. Our data suggest that RNAPII stalled at a DNA lesion triggers a coordinated rescue mechanism that requires the Rad26-Def1 complex, and that Def1 enables ubiquitination and proteolysis of RNAPII when the lesion cannot be rapidly removed by Rad26-promoted DNA repair.
U2 - 10.1038/415929a
DO - 10.1038/415929a
M3 - Journal article
C2 - 11859374
AN - SCOPUS:0037148786
VL - 415
SP - 929
EP - 933
JO - Nature
JF - Nature
SN - 0028-0836
IS - 6874
ER -
ID: 331041945