A novel high-throughput screening method for identifying compounds that inhibit plasmid conjugation

Research output: Contribution to journal › Journal article › Research › peer-review

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A novel high-throughput screening method for identifying compounds that inhibit plasmid conjugation. / Moussa, Jennifer; Gargallo-Viola, Domingo; Thomsen, Line Elnif.

In: MethodsX, Vol. 12, 102740, 2024.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Moussa, J, Gargallo-Viola, D & Thomsen, LE 2024, 'A novel high-throughput screening method for identifying compounds that inhibit plasmid conjugation', MethodsX, vol. 12, 102740. https://doi.org/10.1016/j.mex.2024.102740

APA

Moussa, J., Gargallo-Viola, D., & Thomsen, L. E. (2024). A novel high-throughput screening method for identifying compounds that inhibit plasmid conjugation. MethodsX, 12, [102740]. https://doi.org/10.1016/j.mex.2024.102740

Vancouver

Moussa J, Gargallo-Viola D, Thomsen LE. A novel high-throughput screening method for identifying compounds that inhibit plasmid conjugation. MethodsX. 2024;12. 102740. https://doi.org/10.1016/j.mex.2024.102740

Author

Moussa, Jennifer ; Gargallo-Viola, Domingo ; Thomsen, Line Elnif. / A novel high-throughput screening method for identifying compounds that inhibit plasmid conjugation. In: MethodsX. 2024 ; Vol. 12.

Bibtex

@article{bbc0014b40b14b47853a45d2828d210a,
title = "A novel high-throughput screening method for identifying compounds that inhibit plasmid conjugation",
abstract = "Plasmid conjugation is an important contributing factor to the spread of antibiotic resistance among bacteria, posing a significant global health threat. Our method introduces an innovative high-throughput screening approach to identify compounds that inhibit or reduce conjugation, addressing the need for new strategies against the spread of antimicrobial resistance. Using Escherichia coli strains as donor and recipient, we screened 3500 compounds from a library provided by ABAC Therapeutics. Each 96 –well plate was loaded with 88 different compounds and bacterial cultures. Every plate also included negative and positive controls of conjugation. After an hour, cultures from wells were spotted on agar plates and assessed visually. Compounds that showed a visible effect on conjugation were retested. Six compounds targeting conjugation were found, showing promise for further analysis.",
keywords = "Compound library, Escherichia coli, Plasmid conjugation",
author = "Jennifer Moussa and Domingo Gargallo-Viola and Thomsen, {Line Elnif}",
note = "Publisher Copyright: {\textcopyright} 2024 The Authors",
year = "2024",
doi = "10.1016/j.mex.2024.102740",
language = "English",
volume = "12",
journal = "MethodsX",
issn = "2215-0161",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - A novel high-throughput screening method for identifying compounds that inhibit plasmid conjugation

AU - Moussa, Jennifer

AU - Gargallo-Viola, Domingo

AU - Thomsen, Line Elnif

N1 - Publisher Copyright: © 2024 The Authors

PY - 2024

Y1 - 2024

N2 - Plasmid conjugation is an important contributing factor to the spread of antibiotic resistance among bacteria, posing a significant global health threat. Our method introduces an innovative high-throughput screening approach to identify compounds that inhibit or reduce conjugation, addressing the need for new strategies against the spread of antimicrobial resistance. Using Escherichia coli strains as donor and recipient, we screened 3500 compounds from a library provided by ABAC Therapeutics. Each 96 –well plate was loaded with 88 different compounds and bacterial cultures. Every plate also included negative and positive controls of conjugation. After an hour, cultures from wells were spotted on agar plates and assessed visually. Compounds that showed a visible effect on conjugation were retested. Six compounds targeting conjugation were found, showing promise for further analysis.

AB - Plasmid conjugation is an important contributing factor to the spread of antibiotic resistance among bacteria, posing a significant global health threat. Our method introduces an innovative high-throughput screening approach to identify compounds that inhibit or reduce conjugation, addressing the need for new strategies against the spread of antimicrobial resistance. Using Escherichia coli strains as donor and recipient, we screened 3500 compounds from a library provided by ABAC Therapeutics. Each 96 –well plate was loaded with 88 different compounds and bacterial cultures. Every plate also included negative and positive controls of conjugation. After an hour, cultures from wells were spotted on agar plates and assessed visually. Compounds that showed a visible effect on conjugation were retested. Six compounds targeting conjugation were found, showing promise for further analysis.

KW - Compound library

KW - Escherichia coli

KW - Plasmid conjugation

U2 - 10.1016/j.mex.2024.102740

DO - 10.1016/j.mex.2024.102740

M3 - Journal article

C2 - 38737486

AN - SCOPUS:85192056916

VL - 12

JO - MethodsX

JF - MethodsX

SN - 2215-0161

M1 - 102740

ER -

ID: 394534475