pH dependence of MHC class I-restricted peptide presentation.
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pH dependence of MHC class I-restricted peptide presentation. / Stryhn, A; Pedersen, L O; Romme, T; Olsen, A C; Nissen, Mogens Holst; Thorpe, C J; Buus, S.
I: Journal of Immunology, Bind 156, Nr. 11, 1996, s. 4191-7.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - pH dependence of MHC class I-restricted peptide presentation.
AU - Stryhn, A
AU - Pedersen, L O
AU - Romme, T
AU - Olsen, A C
AU - Nissen, Mogens Holst
AU - Thorpe, C J
AU - Buus, S
N1 - Keywords: Amino Acid Sequence; Animals; Antigen Presentation; Histocompatibility Antigens Class I; Hybridomas; Hydrogen-Ion Concentration; Kinetics; Mice; Molecular Sequence Data; Peptides; Protein Binding; T-Lymphocytes, Cytotoxic; Tumor Cells, Cultured
PY - 1996
Y1 - 1996
N2 - The function of MHC class I molecules is to bind and present antigenic peptides to cytotoxic T cells. Here, we report that class I-restricted peptide presentation is strongly pH dependent. The presentation of some peptides was enhanced at acidic pH, whereas the presentation of others was inhibited. Biochemical peptide-MHC class I binding assays demonstrated that peptide-MHC class I complexes are more stable at neutral pH than at acidic pH. We suggest that acid-dependent peptide dissociation can generate empty class I molecules and that the resulting binding potential can be exploited by a subset of peptide-MHC class I combinations, in some cases leading to considerable peptide exchange. We further speculate that the relative instability of peptide-class I complexes under acidic conditions may affect the outcome of class I-restricted Ag presentation, as less stably associated peptides may dissociate from class I during passage of the acidic trans-Golgi network, and therefore may not be presented. Finally, our results may in part explain how endocytosed proteins can be presented by MHC class I molecules to cytotoxic T cells.
AB - The function of MHC class I molecules is to bind and present antigenic peptides to cytotoxic T cells. Here, we report that class I-restricted peptide presentation is strongly pH dependent. The presentation of some peptides was enhanced at acidic pH, whereas the presentation of others was inhibited. Biochemical peptide-MHC class I binding assays demonstrated that peptide-MHC class I complexes are more stable at neutral pH than at acidic pH. We suggest that acid-dependent peptide dissociation can generate empty class I molecules and that the resulting binding potential can be exploited by a subset of peptide-MHC class I combinations, in some cases leading to considerable peptide exchange. We further speculate that the relative instability of peptide-class I complexes under acidic conditions may affect the outcome of class I-restricted Ag presentation, as less stably associated peptides may dissociate from class I during passage of the acidic trans-Golgi network, and therefore may not be presented. Finally, our results may in part explain how endocytosed proteins can be presented by MHC class I molecules to cytotoxic T cells.
M3 - Journal article
C2 - 8666787
VL - 156
SP - 4191
EP - 4197
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 11
ER -
ID: 8746588