An Optimized Shotgun Strategy for the Rapid Generation of Comprehensive Human Proteomes

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt


This study investigates the challenge of comprehensively cataloging the complete human proteome from a single-cell type using mass spectrometry (MS)-based shotgun proteomics. We modify a classical two-dimensional high-resolution reversed-phase peptide fractionation scheme and optimize a protocol that provides sufficient peak capacity to saturate the sequencing speed of modern MS instruments. This strategy enables the deepest proteome of a human single-cell type to date, with the HeLa proteome sequenced to a depth of ∼584,000 unique peptide sequences and ∼14,200 protein isoforms (∼12,200 protein-coding genes). This depth is comparable with next-generation RNA sequencing and enables the identification of post-translational modifications, including ∼7,000 N-acetylation sites and ∼10,000 phosphorylation sites, without the need for enrichment. We further demonstrate the general applicability and clinical potential of this proteomics strategy by comprehensively quantifying global proteome expression in several different human cancer cell lines and patient tissue samples.

TidsskriftCell Systems
Udgave nummer6
Sider (fra-til)587-599, e1-e4
Antal sider18
StatusUdgivet - 2017

Antal downloads er baseret på statistik fra Google Scholar og

Ingen data tilgængelig

ID: 184291218