Voltage-gated lipid ion channels
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Voltage-gated lipid ion channels. / Blicher, Andreas; Heimburg, Thomas Rainer.
I: P L o S One, Bind 8, Nr. 6, e65707, 2013.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Voltage-gated lipid ion channels
AU - Blicher, Andreas
AU - Heimburg, Thomas Rainer
PY - 2013
Y1 - 2013
N2 - Synthetic lipid membranes can display channel-like ion conduction events even in the absence of proteins. We show here that these events are voltage-gated with a quadratic voltage dependence as expected from electrostatic theory of capacitors. To this end, we recorded channel traces and current histograms in patch-experiments on lipid membranes. We derived a theoretical current-voltage relationship for pores in lipid membranes that describes the experimental data very well when assuming an asymmetric membrane. We determined the equilibrium constant between closed and open state and the open probability as a function of voltage. The voltage-dependence of the lipid pores is found comparable to that of protein channels. Lifetime distributions of open and closed events indicate that the channel open distribution does not follow exponential statistics but rather power law behavior for long open times. Figures
AB - Synthetic lipid membranes can display channel-like ion conduction events even in the absence of proteins. We show here that these events are voltage-gated with a quadratic voltage dependence as expected from electrostatic theory of capacitors. To this end, we recorded channel traces and current histograms in patch-experiments on lipid membranes. We derived a theoretical current-voltage relationship for pores in lipid membranes that describes the experimental data very well when assuming an asymmetric membrane. We determined the equilibrium constant between closed and open state and the open probability as a function of voltage. The voltage-dependence of the lipid pores is found comparable to that of protein channels. Lifetime distributions of open and closed events indicate that the channel open distribution does not follow exponential statistics but rather power law behavior for long open times. Figures
U2 - 10.1371/journal.pone.0065707
DO - 10.1371/journal.pone.0065707
M3 - Journal article
C2 - 23823188
VL - 8
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 6
M1 - e65707
ER -
ID: 46003055