Untargeted metabolomics as a screening tool for estimating compliance to a dietary pattern
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Untargeted metabolomics as a screening tool for estimating compliance to a dietary pattern. / Andersen, Maj-Britt Schmidt; Rinnan, Åsmund; Manach, Claudine; Poulsen, Sanne Kellebjerg; Pujos-Guillot, Estelle; Larsen, Thomas Meinert; Astrup, Arne; Dragsted, Lars Ove.
I: Journal of Proteome Research, Bind 13, Nr. 3, 2014, s. 1405-1418.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Untargeted metabolomics as a screening tool for estimating compliance to a dietary pattern
AU - Andersen, Maj-Britt Schmidt
AU - Rinnan, Åsmund
AU - Manach, Claudine
AU - Poulsen, Sanne Kellebjerg
AU - Pujos-Guillot, Estelle
AU - Larsen, Thomas Meinert
AU - Astrup, Arne
AU - Dragsted, Lars Ove
N1 - CURIS 2014 NEXS 028
PY - 2014
Y1 - 2014
N2 - There is a growing interest in studying the nutritional effects of complex diets. For such studies measurement of dietary compliance is a challenge since the currently available compliance markers only cover limited aspects of a diet. In the present study, an untargeted metabolomics approach was used to develop a compliance measure in urine to distinguish between two dietary patterns. A parallel intervention study was carried out in which 181 participants were randomized to follow either a New Nordic Diet (NND) or an Average Danish Diet (ADD) for six months. Dietary intakes were closely monitored over the whole study period and 24 h urine samples as well as weighed dietary records were collected several times during the study. The urine samples were analysed by UPLC-qTOF-MS and a partial least squares discriminant analysis with feature selection was applied to develop a compliance model based on data from 214 urine samples. The optimised model included fifty-two metabolites and had a misclassification rate of 19 % in a validation set containing 139 samples. The metabolites identified in the model were markers of individual foods such as citrus, cocoa containing products and fish as well as more general dietary traits such as high fruit and vegetable intake or high intake of heat-treated foods. It was easier to classify the ADD diet than the NND diet probably due to seasonal variation in the food composition of NND and indications of lower compliance among the NND subjects. Untargeted metabolomics is a promising approach to develop compliance measures that cover the most important discriminant metabolites of complex diets.
AB - There is a growing interest in studying the nutritional effects of complex diets. For such studies measurement of dietary compliance is a challenge since the currently available compliance markers only cover limited aspects of a diet. In the present study, an untargeted metabolomics approach was used to develop a compliance measure in urine to distinguish between two dietary patterns. A parallel intervention study was carried out in which 181 participants were randomized to follow either a New Nordic Diet (NND) or an Average Danish Diet (ADD) for six months. Dietary intakes were closely monitored over the whole study period and 24 h urine samples as well as weighed dietary records were collected several times during the study. The urine samples were analysed by UPLC-qTOF-MS and a partial least squares discriminant analysis with feature selection was applied to develop a compliance model based on data from 214 urine samples. The optimised model included fifty-two metabolites and had a misclassification rate of 19 % in a validation set containing 139 samples. The metabolites identified in the model were markers of individual foods such as citrus, cocoa containing products and fish as well as more general dietary traits such as high fruit and vegetable intake or high intake of heat-treated foods. It was easier to classify the ADD diet than the NND diet probably due to seasonal variation in the food composition of NND and indications of lower compliance among the NND subjects. Untargeted metabolomics is a promising approach to develop compliance measures that cover the most important discriminant metabolites of complex diets.
U2 - 10.1021/pr400964s
DO - 10.1021/pr400964s
M3 - Journal article
C2 - 24444418
VL - 13
SP - 1405
EP - 1418
JO - Journal of Proteome Research
JF - Journal of Proteome Research
SN - 1535-3893
IS - 3
ER -
ID: 96390598