UBAP2/UBAP2L regulate UV-induced ubiquitylation of RNA polymerase II and are the human orthologues of yeast Def1

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Standard

UBAP2/UBAP2L regulate UV-induced ubiquitylation of RNA polymerase II and are the human orthologues of yeast Def1. / Herlihy, Anna E.; Boeing, Stefan; Weems, Juston C.; Walker, Jane; Dirac-Svejstrup, A. Barbara; Lehner, Michelle Harreman; Conaway, Ronald C.; Conaway, Joan W.; Svejstrup, Jesper Q.

I: DNA Repair, Bind 115, 103343, 2022.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Herlihy, AE, Boeing, S, Weems, JC, Walker, J, Dirac-Svejstrup, AB, Lehner, MH, Conaway, RC, Conaway, JW & Svejstrup, JQ 2022, 'UBAP2/UBAP2L regulate UV-induced ubiquitylation of RNA polymerase II and are the human orthologues of yeast Def1', DNA Repair, bind 115, 103343. https://doi.org/10.1016/j.dnarep.2022.103343

APA

Herlihy, A. E., Boeing, S., Weems, J. C., Walker, J., Dirac-Svejstrup, A. B., Lehner, M. H., Conaway, R. C., Conaway, J. W., & Svejstrup, J. Q. (2022). UBAP2/UBAP2L regulate UV-induced ubiquitylation of RNA polymerase II and are the human orthologues of yeast Def1. DNA Repair, 115, [103343]. https://doi.org/10.1016/j.dnarep.2022.103343

Vancouver

Herlihy AE, Boeing S, Weems JC, Walker J, Dirac-Svejstrup AB, Lehner MH o.a. UBAP2/UBAP2L regulate UV-induced ubiquitylation of RNA polymerase II and are the human orthologues of yeast Def1. DNA Repair. 2022;115. 103343. https://doi.org/10.1016/j.dnarep.2022.103343

Author

Herlihy, Anna E. ; Boeing, Stefan ; Weems, Juston C. ; Walker, Jane ; Dirac-Svejstrup, A. Barbara ; Lehner, Michelle Harreman ; Conaway, Ronald C. ; Conaway, Joan W. ; Svejstrup, Jesper Q. / UBAP2/UBAP2L regulate UV-induced ubiquitylation of RNA polymerase II and are the human orthologues of yeast Def1. I: DNA Repair. 2022 ; Bind 115.

Bibtex

@article{f1812ba6b3ca4c69a33d66938049ea43,

title = "UBAP2/UBAP2L regulate UV-induced ubiquitylation of RNA polymerase II and are the human orthologues of yeast Def1",

abstract = "During transcription, RNA polymerase II (RNAPII) faces numerous obstacles, including DNA damage, which can lead to stalling or arrest. One mechanism to contend with this situation is ubiquitylation and degradation of the largest RNAPII subunit, RPB1 - the {\textquoteleft}last resort{\textquoteright} pathway. This conserved, multi-step pathway was first identified in yeast, and the functional human orthologues of all but one protein, RNAPII Degradation Factor 1 (Def1), have been discovered. Here we show that following UV-irradiation, human Ubiquitin-associated protein 2 (UBAP2) or its paralogue UBAP2-like (UBAP2L) are involved in the ubiquitylation and degradation of RNAPII through the recruitment of Elongin-Cul5 ubiquitin ligase. Together, our data indicate that UBAP2 and UBAP2L are the human orthologues of yeast Def1, and so identify the key missing proteins in the human last resort pathway.",

keywords = "Def1, RNA polymerase II ubiquitylation, Transcription-coupled nucleotide excision repair, UBAP2, UBAP2L, Ubiquitin",

author = "Herlihy, {Anna E.} and Stefan Boeing and Weems, {Juston C.} and Jane Walker and Dirac-Svejstrup, {A. Barbara} and Lehner, {Michelle Harreman} and Conaway, {Ronald C.} and Conaway, {Joan W.} and Svejstrup, {Jesper Q.}",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2022",

doi = "10.1016/j.dnarep.2022.103343",

language = "English",

volume = "115",

journal = "DNA Repair",

issn = "1568-7864",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - UBAP2/UBAP2L regulate UV-induced ubiquitylation of RNA polymerase II and are the human orthologues of yeast Def1

AU - Herlihy, Anna E.

AU - Boeing, Stefan

AU - Weems, Juston C.

AU - Walker, Jane

AU - Dirac-Svejstrup, A. Barbara

AU - Lehner, Michelle Harreman

AU - Conaway, Ronald C.

AU - Conaway, Joan W.

AU - Svejstrup, Jesper Q.

PY - 2022

Y1 - 2022

N2 - During transcription, RNA polymerase II (RNAPII) faces numerous obstacles, including DNA damage, which can lead to stalling or arrest. One mechanism to contend with this situation is ubiquitylation and degradation of the largest RNAPII subunit, RPB1 - the ‘last resort’ pathway. This conserved, multi-step pathway was first identified in yeast, and the functional human orthologues of all but one protein, RNAPII Degradation Factor 1 (Def1), have been discovered. Here we show that following UV-irradiation, human Ubiquitin-associated protein 2 (UBAP2) or its paralogue UBAP2-like (UBAP2L) are involved in the ubiquitylation and degradation of RNAPII through the recruitment of Elongin-Cul5 ubiquitin ligase. Together, our data indicate that UBAP2 and UBAP2L are the human orthologues of yeast Def1, and so identify the key missing proteins in the human last resort pathway.

AB - During transcription, RNA polymerase II (RNAPII) faces numerous obstacles, including DNA damage, which can lead to stalling or arrest. One mechanism to contend with this situation is ubiquitylation and degradation of the largest RNAPII subunit, RPB1 - the ‘last resort’ pathway. This conserved, multi-step pathway was first identified in yeast, and the functional human orthologues of all but one protein, RNAPII Degradation Factor 1 (Def1), have been discovered. Here we show that following UV-irradiation, human Ubiquitin-associated protein 2 (UBAP2) or its paralogue UBAP2-like (UBAP2L) are involved in the ubiquitylation and degradation of RNAPII through the recruitment of Elongin-Cul5 ubiquitin ligase. Together, our data indicate that UBAP2 and UBAP2L are the human orthologues of yeast Def1, and so identify the key missing proteins in the human last resort pathway.

KW - Def1

KW - RNA polymerase II ubiquitylation

KW - Transcription-coupled nucleotide excision repair

KW - UBAP2

KW - UBAP2L

KW - Ubiquitin

U2 - 10.1016/j.dnarep.2022.103343

DO - 10.1016/j.dnarep.2022.103343

M3 - Journal article

C2 - 35633597

AN - SCOPUS:85131052968

VL - 115

JO - DNA Repair

JF - DNA Repair

SN - 1568-7864

M1 - 103343

ER -

ID: 313479462