Tumor-associated antigens identified by mRNA expression profiling induce protective anti-tumor immunity
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Tumor-associated antigens identified by mRNA expression profiling induce protective anti-tumor immunity. / Mathiassen, S; Lauemøller, S L; Ruhwald, M; Claesson, M H; Buus, S.
I: European Journal of Immunology, Bind 31, Nr. 4, 2001, s. 1239-46.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Tumor-associated antigens identified by mRNA expression profiling induce protective anti-tumor immunity
AU - Mathiassen, S
AU - Lauemøller, S L
AU - Ruhwald, M
AU - Claesson, M H
AU - Buus, S
N1 - Keywords: Amino Acid Sequence; Animals; Antigens, Neoplasm; Autoantigens; Cancer Vaccines; Cytotoxicity, Immunologic; DNA, Complementary; DNA-Binding Proteins; Epitopes, T-Lymphocyte; Fungal Proteins; Gene Deletion; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Genes, p53; H-2 Antigens; Mice; Mice, Inbred C57BL; Mice, Knockout; Neoplasm Transplantation; Organ Specificity; Peptides; RNA, Messenger; Saccharomyces cerevisiae Proteins; Survival Rate; Thymoma; Tumor Cells, Cultured; Vaccination
PY - 2001
Y1 - 2001
N2 - Defined tumor-associated antigens (TAA) are attractive targets for anti-tumor immunotherapy. Here, we describe a novel genome-wide approach to identify multiple TAA from any given tumor. A panel of transplantable thymomas was established from an inbred p53-/- mouse strain. The resulting tumors were examined for gene expression by mRNA microarray scanning. This analysis revealed heterogeneity of the tumors in agreement with the assumption that they represent different tumorigenic events. Several genes were overexpressed in one or more of the tumors. To examine whether overexpressed genes might be used to identify TAA, mice were immunized with mixtures of peptides representing putative cytotoxic T cell epitopes derived from one of the gene products. Indeed, such immunized mice were partially protected against subsequent tumor challenge. Despite being immunized with bona fide self antigens, no clinical signs of autoimmune reactions were observed. Thus, it appears possible to evaluate the entire metabolism of any given tumor and use this information rationally to identify multiple epitopes of value in the generation of tumor-specific immunotherapy. We expect that human tumors express similar tumor-specific metabolic imprints, which may be used to identify patient-specific arrays of TAA. This may enable a multi-epitope based immunotherapy with improved prospects of clinical tumor rejection.
AB - Defined tumor-associated antigens (TAA) are attractive targets for anti-tumor immunotherapy. Here, we describe a novel genome-wide approach to identify multiple TAA from any given tumor. A panel of transplantable thymomas was established from an inbred p53-/- mouse strain. The resulting tumors were examined for gene expression by mRNA microarray scanning. This analysis revealed heterogeneity of the tumors in agreement with the assumption that they represent different tumorigenic events. Several genes were overexpressed in one or more of the tumors. To examine whether overexpressed genes might be used to identify TAA, mice were immunized with mixtures of peptides representing putative cytotoxic T cell epitopes derived from one of the gene products. Indeed, such immunized mice were partially protected against subsequent tumor challenge. Despite being immunized with bona fide self antigens, no clinical signs of autoimmune reactions were observed. Thus, it appears possible to evaluate the entire metabolism of any given tumor and use this information rationally to identify multiple epitopes of value in the generation of tumor-specific immunotherapy. We expect that human tumors express similar tumor-specific metabolic imprints, which may be used to identify patient-specific arrays of TAA. This may enable a multi-epitope based immunotherapy with improved prospects of clinical tumor rejection.
M3 - Journal article
C2 - 11298350
VL - 31
SP - 1239
EP - 1246
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 4
ER -
ID: 9944297