Tumor associated antigen specific T-cell populations identified in ex vivo expanded TIL cultures
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Tumor associated antigen specific T-cell populations identified in ex vivo expanded TIL cultures. / Junker, Niels; Kvistborg, Pia; Køllgaard, Tania; Straten, Per thor; Andersen, Mads Hald; Svane, Inge Marie.
I: Cellular Immunology, Bind 273, Nr. 1, 2012, s. 1-9.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Tumor associated antigen specific T-cell populations identified in ex vivo expanded TIL cultures
AU - Junker, Niels
AU - Kvistborg, Pia
AU - Køllgaard, Tania
AU - Straten, Per thor
AU - Andersen, Mads Hald
AU - Svane, Inge Marie
N1 - Copyright © 2011 Elsevier Inc. All rights reserved.
PY - 2012
Y1 - 2012
N2 - Ex vivo expanded tumor infiltrating lymphocytes (TILs) from malignant melanoma (MM) and head & neck squamous cell carcinoma (HNSCC) share a similar oligoclonal composition of T effector memory cells, with HLA class I restricted lysis of tumor cell lines. In this study we show that ex vivo expanded TILs from MM and HNSCC demonstrate a heterogeneous composition in frequency and magnitude of tumor associated antigen specific populations by Elispot IFN¿ quantitation. TILs from MM and HNSCC shared reactivity towards NY ESO-1, cyclin B1 and Bcl-x derived peptides. Additionally we show that dominating T-cell clones and functionality persists through out expansion among an oligoclonal composition of T-cells. Our findings mirror prior results on the oligoclonal composition of TIL cultures, further indicating a potential for a broader repertoire of specific effector cells recognizing the heterogeneous tumors upon adoptive transfer; increasing the probability of tumor control by minimizing immune evasion by tumor cell escape variants.
AB - Ex vivo expanded tumor infiltrating lymphocytes (TILs) from malignant melanoma (MM) and head & neck squamous cell carcinoma (HNSCC) share a similar oligoclonal composition of T effector memory cells, with HLA class I restricted lysis of tumor cell lines. In this study we show that ex vivo expanded TILs from MM and HNSCC demonstrate a heterogeneous composition in frequency and magnitude of tumor associated antigen specific populations by Elispot IFN¿ quantitation. TILs from MM and HNSCC shared reactivity towards NY ESO-1, cyclin B1 and Bcl-x derived peptides. Additionally we show that dominating T-cell clones and functionality persists through out expansion among an oligoclonal composition of T-cells. Our findings mirror prior results on the oligoclonal composition of TIL cultures, further indicating a potential for a broader repertoire of specific effector cells recognizing the heterogeneous tumors upon adoptive transfer; increasing the probability of tumor control by minimizing immune evasion by tumor cell escape variants.
U2 - 10.1016/j.cellimm.2011.12.004
DO - 10.1016/j.cellimm.2011.12.004
M3 - Journal article
C2 - 22230732
VL - 273
SP - 1
EP - 9
JO - Cellular Immunology
JF - Cellular Immunology
SN - 0008-8749
IS - 1
ER -
ID: 40196979