Transfection of tumor-infiltrating T cells with mRNA encoding CXCR2
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Transfection of tumor-infiltrating T cells with mRNA encoding CXCR2. / Idorn, Manja; thor Straten, Eivind Per; Svane, Inge Marie; Met, Özcan.
Synthetic mRNA: Production, Introduction Into Cells, and Physiological Consequences. red. / Robert E. Rhoads. Bind 1428 Springer, 2016. s. 261-276 (Methods in Molecular Biology, Bind 1428).Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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TY - CHAP
T1 - Transfection of tumor-infiltrating T cells with mRNA encoding CXCR2
AU - Idorn, Manja
AU - thor Straten, Eivind Per
AU - Svane, Inge Marie
AU - Met, Özcan
PY - 2016
Y1 - 2016
N2 - Adoptive T-cell therapy based on the infusion of patient’s own immune cells after ex vivo culturing is among the most potent forms of personalized treatment among recent clinical developments for the treatment of cancer. However, despite high rates of successful initial clinical responses, only about 20 % of patients with metastatic melanoma treated with tumor-infiltrating lymphocytes (TILs) enter complete and long-term regression, with the majority either relapsing after initial partial regression or not benefiting at all. Previous studies have shown a positive correlation between the number infused T cells migrating to the tumor and the clinical response, but also that only a small fraction of adoptively transferred Tcells reach the tumor site. In this chapter, we describe a protocol for transfection of TILs with mRNA encoding the chemokine receptor CXCR2 transiently redirecting and improving TILs migration toward tumor-secreted chemokines in vitro.
AB - Adoptive T-cell therapy based on the infusion of patient’s own immune cells after ex vivo culturing is among the most potent forms of personalized treatment among recent clinical developments for the treatment of cancer. However, despite high rates of successful initial clinical responses, only about 20 % of patients with metastatic melanoma treated with tumor-infiltrating lymphocytes (TILs) enter complete and long-term regression, with the majority either relapsing after initial partial regression or not benefiting at all. Previous studies have shown a positive correlation between the number infused T cells migrating to the tumor and the clinical response, but also that only a small fraction of adoptively transferred Tcells reach the tumor site. In this chapter, we describe a protocol for transfection of TILs with mRNA encoding the chemokine receptor CXCR2 transiently redirecting and improving TILs migration toward tumor-secreted chemokines in vitro.
KW - Cancer immunotherapy
KW - Chemokine receptor
KW - mRNA transfection
KW - Tumor homing
KW - Tumor-infiltrating lymphocytes (TILs)
U2 - 10.1007/978-1-4939-3625-0_17
DO - 10.1007/978-1-4939-3625-0_17
M3 - Book chapter
C2 - 27236805
AN - SCOPUS:84990194133
SN - 978-1-4939-3623-6
VL - 1428
T3 - Methods in Molecular Biology
SP - 261
EP - 276
BT - Synthetic mRNA
A2 - Rhoads, Robert E.
PB - Springer
ER -
ID: 176375764