Transcriptomic and lipid profiling analysis reveals a functional interplay between testosterone and growth hormone in hypothyroid liver
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Dokumenter
- Fulltext
Forlagets udgivne version, 3,82 MB, PDF-dokument
Preclinical and clinical studies suggest that hypothyroidism might cause hepatic endocrine and metabolic disturbances with features that mimic deficiencies of testosterone and/or GH. The absence of physiological interactions between testosterone and GH can be linked to male differentiated liver diseases. Testosterone plays relevant physiological effects on somatotropic-liver axis and liver composition and the liver is a primary organ of interactions between testosterone and GH. However, testosterone exerts many effects on liver through complex and poorly understood mechanisms. Testosterone impacts liver functions by binding to the Androgen Receptor, and, indirectly, through its conversion to estradiol, and cooperation with GH. However, the role of testosterone, and its interaction with GH, in the hypothyroid liver, remains unclear. In the present work, the effects of testosterone, and how they impact on GH-regulated whole transcriptome and lipid composition in the liver, were studied in the context of adult hypothyroid-orchiectomized rats. Testosterone replacement positively modulated somatotropic-liver axis and impacted liver transcriptome involved in lipid and glucose metabolism. In addition, testosterone enhanced the effects of GH on the transcriptome linked to lipid biosynthesis, oxidation-reduction, and metabolism of unsaturated and long-chain fatty acids (FA). However, testosterone decreased the hepatic content of cholesterol esters and triacylglycerols and increased fatty acids whereas GH increased neutral lipids and decreased polar lipids. Biological network analysis of the effects of testosterone on GH-regulated transcriptome confirmed a close connection with crucial proteins involved in steroid and fatty acid metabolism. Taken together, this comprehensive analysis of gene expression and lipid profiling in hypothyroid male liver reveals a functional interplay between testosterone and pulsed GH administration.
Originalsprog | Engelsk |
---|---|
Artikelnummer | 1266150 |
Tidsskrift | Frontiers in Endocrinology |
Vol/bind | 14 |
Antal sider | 24 |
ISSN | 1664-2392 |
DOI | |
Status | Udgivet - 2023 |
Bibliografisk note
Funding Information:
The author(s) declare financial support was received for the research, authorship, and/or publication of this article. The Spanish Ministry of Science and Innovation with the funding of European Regional Development Fund-European Social Fund supported this research by grants-in-aid to Molecular and Translational Pharmacology Group-IUIBS (SAF2012-37344-C03-02; SAF2015-65113-C2-2) and MD (SAF2014-52582-R). Molecular and Translational Pharmacology Group was also supported by grants-in-aid from Arehucas Foundation - Canary Islands Foundation for Cancer Research (FICIC). DI-G was supported by grants from the Novo Nordisk Foundation and the Danish Cancer Society. Acknowledgments
Publisher Copyright:
Copyright © 2023 Fernández-Pérez, Guerra, Recio, Cabrera-Galván, García, De La Rosa, Castrillo, Iglesias-Gato and Díaz.
ID: 378947265