Transcriptional inhibition of genes with severe histone H3 hypoacetylation in the coding region
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Transcriptional inhibition of genes with severe histone H3 hypoacetylation in the coding region. / Kristjuhan, Arnold; Walker, Jane; Suka, Noriyuki; Grunstein, Michael; Roberts, Douglas; Cairns, Bradley R.; Svejstrup, Jesper Q.
I: Molecular Cell, Bind 10, Nr. 4, 01.10.2002, s. 925-933.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Transcriptional inhibition of genes with severe histone H3 hypoacetylation in the coding region
AU - Kristjuhan, Arnold
AU - Walker, Jane
AU - Suka, Noriyuki
AU - Grunstein, Michael
AU - Roberts, Douglas
AU - Cairns, Bradley R.
AU - Svejstrup, Jesper Q.
N1 - Funding Information: This work was supported by grants from Cancer Research UK and the EU (HPRN-CT-2000-00087) to J.Q.S., by Public Health Service Grant of the National Institutes of Health (NIH) GM23674 to M.G., and by Public Health Service Grant of the NIH GM60415 and support of core facilities by NIH (CA24014) to B.R.C. We thank Kevin Struhl and Alain Verreault for gifts of yeast strains and antibody, respectively. Tomas Lindahl and Alain Verreault are thanked for comments on the manuscript. Cancer Research UK London Research Institute comprises the Lincoln's Inn Fields and Clare Hall Laboratories of the former Imperial Cancer Research Fund following the merger of the ICRF with the Cancer Research Campaign in February 2002.
PY - 2002/10/1
Y1 - 2002/10/1
N2 - Changes in histone acetylation at promoters correlate with transcriptional activation and repression, but whether acetylation of histones in the coding region of genes is important for transcription is less clear. Here, we show that cells lacking the histone acetyltransferases Gcn5 and Elp3 have widespread and severe histone H3 hypoacetylation in chromatin. Surprisingly, severe hypoacetylation in the promoter does not invariably affect the ability of TBP to bind the TATA element, or transcription of the gene. By contrast, similar hypoacetylation of the coding region correlates with inhibition of transcription, and inhibition correlates better with the overall charge of the histone H3 tail than with hypoacetylation of specific lysine residues. These data provide insights into the effects of histone H3 hypoacetylation in vivo and underscore the importance of the overall charge of the histone tail for transcription.
AB - Changes in histone acetylation at promoters correlate with transcriptional activation and repression, but whether acetylation of histones in the coding region of genes is important for transcription is less clear. Here, we show that cells lacking the histone acetyltransferases Gcn5 and Elp3 have widespread and severe histone H3 hypoacetylation in chromatin. Surprisingly, severe hypoacetylation in the promoter does not invariably affect the ability of TBP to bind the TATA element, or transcription of the gene. By contrast, similar hypoacetylation of the coding region correlates with inhibition of transcription, and inhibition correlates better with the overall charge of the histone H3 tail than with hypoacetylation of specific lysine residues. These data provide insights into the effects of histone H3 hypoacetylation in vivo and underscore the importance of the overall charge of the histone tail for transcription.
UR - http://www.scopus.com/inward/record.url?scp=0036809640&partnerID=8YFLogxK
U2 - 10.1016/S1097-2765(02)00647-0
DO - 10.1016/S1097-2765(02)00647-0
M3 - Journal article
C2 - 12419235
AN - SCOPUS:0036809640
VL - 10
SP - 925
EP - 933
JO - Molecular Cell
JF - Molecular Cell
SN - 1097-2765
IS - 4
ER -
ID: 331042360