Transcranial photoacoustic imaging of NMDA-evoked focal circuit dynamics in the rat hippocampus
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Transcranial photoacoustic imaging of NMDA-evoked focal circuit dynamics in the rat hippocampus. / Kang, Jeeun; Kadam, Shilpa D.; Elmore, Joshua S.; Sullivan, Brennan J.; Valentine, Heather; Malla, Adarsha P.; Harraz, Maged M.; Rahmim, Arman; Kang, Jin U.; Loew, Leslie M.; Baumann, Michael H.; Grace, Anthony A.; Gjedde, Albert; Boctor, Emad M.; Wong, Dean F.
I: Journal of Neural Engineering, Bind 17, Nr. 2, 025001, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Transcranial photoacoustic imaging of NMDA-evoked focal circuit dynamics in the rat hippocampus
AU - Kang, Jeeun
AU - Kadam, Shilpa D.
AU - Elmore, Joshua S.
AU - Sullivan, Brennan J.
AU - Valentine, Heather
AU - Malla, Adarsha P.
AU - Harraz, Maged M.
AU - Rahmim, Arman
AU - Kang, Jin U.
AU - Loew, Leslie M.
AU - Baumann, Michael H.
AU - Grace, Anthony A.
AU - Gjedde, Albert
AU - Boctor, Emad M.
AU - Wong, Dean F.
PY - 2020
Y1 - 2020
N2 - Objective. We report the transcranial functional photoacoustic (fPA) neuroimaging of N-methyl-D-aspartate (NMDA) evoked neural activity in the rat hippocampus. Concurrent quantitative electroencephalography (qEEG) and microdialysis were used to record real-time circuit dynamics and excitatory neurotransmitter concentrations, respectively.Approach. We hypothesized that location-specific fPA voltage-sensitive dye (VSD) contrast would identify neural activity changes in the hippocampus which correlate with NMDA-evoked excitatory neurotransmission.Main results. Transcranial fPA VSD imaging at the contralateral side of the microdialysis probe provided NMDA-evoked VSD responses with positive correlation to extracellular glutamate concentration changes. qEEG validated a wide range of glutamatergic excitation, which culminated in focal seizure activity after a high NMDA dose. We conclude that transcranial fPA VSD imaging can distinguish focal glutamate loads in the rat hippocampus, based on the VSD redistribution mechanism which is sensitive to the electrophysiologic membrane potential.Significance. Our results suggest the future utility of this emerging technology in both laboratory and clinical sciences as an innovative functional neuroimaging modality.
AB - Objective. We report the transcranial functional photoacoustic (fPA) neuroimaging of N-methyl-D-aspartate (NMDA) evoked neural activity in the rat hippocampus. Concurrent quantitative electroencephalography (qEEG) and microdialysis were used to record real-time circuit dynamics and excitatory neurotransmitter concentrations, respectively.Approach. We hypothesized that location-specific fPA voltage-sensitive dye (VSD) contrast would identify neural activity changes in the hippocampus which correlate with NMDA-evoked excitatory neurotransmission.Main results. Transcranial fPA VSD imaging at the contralateral side of the microdialysis probe provided NMDA-evoked VSD responses with positive correlation to extracellular glutamate concentration changes. qEEG validated a wide range of glutamatergic excitation, which culminated in focal seizure activity after a high NMDA dose. We conclude that transcranial fPA VSD imaging can distinguish focal glutamate loads in the rat hippocampus, based on the VSD redistribution mechanism which is sensitive to the electrophysiologic membrane potential.Significance. Our results suggest the future utility of this emerging technology in both laboratory and clinical sciences as an innovative functional neuroimaging modality.
KW - photoacoustic
KW - voltage-sensitive dye
KW - microdialysis
KW - quantitative electroencephalogram
KW - N-methyl-D-aspartate
KW - glutamate
KW - hippocampus
KW - IN-VIVO
KW - SPATIOTEMPORAL DYNAMICS
KW - TOMOGRAPHY
KW - ULTRASOUND
KW - MICRODIALYSIS
KW - MODEL
KW - RESOLUTION
KW - METHYLONE
KW - SENSOR
KW - FRAMES
U2 - 10.1088/1741-2552/ab78ca
DO - 10.1088/1741-2552/ab78ca
M3 - Journal article
C2 - 32084654
VL - 17
JO - Journal of Neural Engineering
JF - Journal of Neural Engineering
SN - 1741-2560
IS - 2
M1 - 025001
ER -
ID: 248334526